Preparation of differently sized injectable collagen micro-scaffolds.

Authors :: Geutjes PJ
Faraj KA
Daamen WF
van Kuppevelt TH
Source :: Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2011 Aug; Vol. 5 (8), pp. 665-7. Date of Electronic Publication: 2010 Dec 29.
Publication Year :: 2011
Abstract: Collagen scaffolds have been widely used as biomaterials for tissue engineering. In general, application of scaffolds requires surgery. In this study, we describe a new and simple technique to prepare porous micro-scaffolds from type I collagen fibrils which can be injected, thus preventing surgery. The size of the micro-scaffolds could be easily controlled using sieves with varying cut-offs. EDC-NHS crosslinking was essential to stabilize the collagen micro-scaffolds. Micro-scaffolds were highly porous and could be injected through small diameter needles (18-21 gauge). Collagen micro-scaffolds may be used as injectables for the local delivery of effector molecules and/or cells, thus creating specific niches to enhance local tissue regeneration.<br /> (Copyright © 2010 John Wiley & Sons, Ltd.)