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Protective live oral brucellosis vaccines stimulate Th1 and th17 cell responses.
- Source :
-
Infection and immunity [Infect Immun] 2011 Oct; Vol. 79 (10), pp. 4165-74. Date of Electronic Publication: 2011 Jul 18. - Publication Year :
- 2011
-
Abstract
- Zoonotic transmission of brucellosis often results from exposure to Brucella-infected livestock, feral animals, or wildlife or frequently via consumption of unpasteurized milk products or raw meat. Since natural infection of humans often occurs by the oral route, mucosal vaccination may offer a means to confer protection for both mucosal and systemic tissues. Significant efforts have focused on developing a live brucellosis vaccine, and deletion of the znuA gene involved in zinc transport has been found to attenuate Brucella abortus. A similar mutation has been adapted for Brucella melitensis and tested to determine whether oral administration of ΔznuA B. melitensis can confer protection against nasal B. melitensis challenge. A single oral vaccination with ΔznuA B. melitensis rapidly cleared from mice within 2 weeks and effectively protected mice upon nasal challenge with wild-type B. melitensis 16M. In 83% of the vaccinated mice, no detectable brucellae were found in their spleens, unlike with phosphate-buffered saline (PBS)-dosed mice, and vaccination also enhanced the clearance of brucellae from the lungs. Moreover, vaccinated gamma interferon-deficient (IFN-γ(-/-)) mice also showed protection in both spleens and lungs, albeit protection that was not as effective as in immunocompetent mice. Although IFN-γ, interleukin 17 (IL-17), and IL-22 were stimulated by these live vaccines, only RB51-mediated protection was codependent upon IL-17 in BALB/c mice. These data suggest that oral immunization with the live, attenuated ΔznuA B. melitensis vaccine provides an attractive strategy to protect against inhalational infection with virulent B. melitensis.
- Subjects :
- Administration, Oral
Animals
Brucella Vaccine administration & dosage
Brucella Vaccine genetics
Brucella melitensis pathogenicity
Brucellosis prevention & control
Female
Interleukin-17 metabolism
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Vaccination
Vaccines, Attenuated administration & dosage
Vaccines, Attenuated genetics
Brucella Vaccine immunology
Brucella melitensis immunology
Brucellosis immunology
Th1 Cells immunology
Th17 Cells immunology
Vaccines, Attenuated immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5522
- Volume :
- 79
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 21768283
- Full Text :
- https://doi.org/10.1128/IAI.05080-11