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High-dose green tea polyphenols induce nephrotoxicity in dextran sulfate sodium-induced colitis mice by down-regulation of antioxidant enzymes and heat-shock protein expressions.
- Source :
-
Cell stress & chaperones [Cell Stress Chaperones] 2011 Nov; Vol. 16 (6), pp. 653-62. Date of Electronic Publication: 2011 Jul 16. - Publication Year :
- 2011
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Abstract
- Previously, we reported that oral feeding of 1% green tea polyphenols (GTPs) aggravated the dextran sulfate sodium (DSS)-induced colitis in mice. In the present study, we assessed the toxicity of 1% GTPs in several organs from normal and DSS-exposed mice. Sixty-two male ICR mice were initially divided into four groups. Non-treated group (group 1, n = 15) was given standard diet and water, GTPs (group 2, n = 15) received 1% GTPs in diet and water, DSS (group 3, n = 15) received diet and 5% DSS in water, and GTPs + DSS group (group 4, n = 17) received 1% GTPs in diet and 5% DSS in water. We found that group 4 significantly increased (P < 0.05) kidney weight, the levels of serum creatinine and thiobarbituric acid-reactive substances in both kidney and liver, as compared with those in group 3. The mRNA expression levels of antioxidant enzymes and heat-shock proteins (HSPs) in group 4 were lower than those of group 3. For instance, heme oxygenase-1 (HO-1), HSP27, and 90 mRNA in the kidney of group 4 were dramatically down-regulated as compared with those of group 3. Furthermore, 1% GTPs diet decreased the expression of HO-1, NAD(P)H:quinone oxidoreductase 1 (NQO1) and HSP90 in kidney and liver of non-treated mice. Taken together, our results indicate that high-dose GTPs diet disrupts kidney functions through the reduction of antioxidant enzymes and heat-shock protein expressions in not only colitis but also non-treated ICR mice.
- Subjects :
- Animals
Dextran Sulfate
Down-Regulation drug effects
Heme Oxygenase-1 genetics
Kidney metabolism
Lipid Peroxidation drug effects
Liver drug effects
Male
Mice
Organ Size drug effects
Polyphenols administration & dosage
RNA, Messenger genetics
Antioxidants metabolism
Colitis chemically induced
Heat-Shock Proteins genetics
Kidney drug effects
Polyphenols toxicity
Tea toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1466-1268
- Volume :
- 16
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Cell stress & chaperones
- Publication Type :
- Academic Journal
- Accession number :
- 21766215
- Full Text :
- https://doi.org/10.1007/s12192-011-0280-8