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Improved biocompatibility of novel poly(L-lactic acid)/β-tricalcium phosphate scaffolds prepared by an organic solvent-free method.

Authors :
Zhao XF
Li XD
Kang YQ
Yuan Q
Source :
International journal of nanomedicine [Int J Nanomedicine] 2011; Vol. 6, pp. 1385-90. Date of Electronic Publication: 2011 Jul 04.
Publication Year :
2011

Abstract

A porous poly(L-lactic acid)/β-tricalcium phosphate (PLLA/β-TCP) composite scaffold was fabricated using a novel technique comprising powder mixing, compression molding, low-temperature treatment, and particulate leaching without any organic solvent. The effect of this scaffold on osteoblast proliferation and differentiation was evaluated in vitro. The fabricated scaffold had a homogeneously interconnected porous structure with a porosity of 70% and compressive strength of 1.35 MPa. The methylthiazol tetrazolium values and alkaline phosphatase (ALP) activity of osteoblasts seeded on the solvent-free scaffold were significant higher than those of the control. Using real-time PCR, gene expressions of ALP, osteocalcin, and type 1 collagen were shown to be upregulated. As the method does not use any organic solvent, it eliminates problems associated with organic solvent residue and therefore improves the cell compatibility. It has a promising potential for the preparation of porous scaffold for bone tissue engineering.

Details

Language :
English
ISSN :
1178-2013
Volume :
6
Database :
MEDLINE
Journal :
International journal of nanomedicine
Publication Type :
Academic Journal
Accession number :
21760732
Full Text :
https://doi.org/10.2147/IJN.S20743