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Use of sindbis/eastern equine encephalitis chimeric viruses in plaque reduction neutralization tests for arboviral disease diagnostics.
- Source :
-
Clinical and vaccine immunology : CVI [Clin Vaccine Immunol] 2011 Sep; Vol. 18 (9), pp. 1486-91. Date of Electronic Publication: 2011 Jul 13. - Publication Year :
- 2011
-
Abstract
- Eastern equine encephalitis virus (EEEV) is a highly virulent, mosquito-borne alphavirus that causes severe and often fatal neurological disease in humans and horses in eastern North American, the Caribbean, and Mexico and throughout Central and South America. EEEV infection is diagnosed serologically by anti-EEEV-specific IgM detection, with confirmation by the plaque reduction neutralization test (PRNT), which is highly specific for alphaviruses. Live virus is used in the PRNT procedure, which currently requires biosafety level 3 containment facilities and select agent security in the case of EEEV. These requirements restrict the ability of public health laboratories to conduct PRNTs. Sindbis virus (SINV)/EEEV recombinant constructs have been engineered to express the immunogenic structural proteins from 2 wild-type EEEV strains in an attenuated form. These SINV/EEEVs, which are not classified as select agents, were evaluated as alternative diagnostic reagents in a PRNT using human, equine, and murine sera. The results indicate that the chimeric viruses exhibit specificity comparable to that of wild-type EEEV, with only a slight reduction in sensitivity. Considering their benefits in increased safety and reduced regulatory requirements, these chimeric viruses should be highly useful in diagnostic laboratories throughout the Americas.
- Subjects :
- Alphavirus Infections immunology
Alphavirus Infections virology
Animals
Antibodies, Viral blood
Encephalitis Virus, Eastern Equine genetics
Encephalomyelitis, Equine diagnosis
Encephalomyelitis, Equine immunology
Encephalomyelitis, Equine virology
Genetic Engineering
Horse Diseases diagnosis
Horse Diseases immunology
Horse Diseases virology
Horses immunology
Humans
Mice
Sindbis Virus genetics
Viral Structural Proteins genetics
Viral Structural Proteins immunology
Viral Structural Proteins metabolism
Alphavirus Infections diagnosis
Encephalitis Virus, Eastern Equine immunology
Neutralization Tests methods
Recombination, Genetic
Sindbis Virus immunology
Viral Plaque Assay methods
Subjects
Details
- Language :
- English
- ISSN :
- 1556-679X
- Volume :
- 18
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Clinical and vaccine immunology : CVI
- Publication Type :
- Academic Journal
- Accession number :
- 21752946
- Full Text :
- https://doi.org/10.1128/CVI.05129-11