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Proteomics in the liver of gilthead sea bream (Sparus aurata) to elucidate the cellular response induced by the intake of maslinic acid.
- Source :
-
Proteomics [Proteomics] 2011 Aug; Vol. 11 (16), pp. 3312-25. Date of Electronic Publication: 2011 Jul 14. - Publication Year :
- 2011
-
Abstract
- Maslinic acid (MA) is a pentacyclic triterpene used as a feed additive to stimulate growth, protein-turnover rates, and hyperplasia in fish. To further our understanding of cellular mechanisms underlying the action of MA, we have used 2-DE coupled with MS to identify proteins differentially expressed in the livers of juvenile gilthead sea bream (Sparus aurata) grown under fish-farm conditions and fed with a 100 mg/kg MA-enriched diet (MA(100)). After the comparison of the protein profiles from MA(100) fed fish and from control, 49 protein spots were found to be altered in abundance (≥2-fold). Analysis by MALDI-TOF/TOF allowed the unambiguous identification of 29 spots, corresponding to 19 different proteins. These proteins were: phosphoglucomutase, phosphoglucose isomerase, S-adenosyl methionine-dependent methyltransferase class I, aldehyde dehydrogenase, catalase, 6-phosphogluconate dehydrogenase, fumarylacetoacetate hydrolase, 4-hydroxyphenylpyruvic dioxygenase, methylmalonate-semialdehyde dehydrogenase, lysozyme, urate oxidase, elongation factor 2, 60 kDa heat-shock protein, 58 kDa glucose-regulated protein, cytokeratin E7, type-II keratin, intermediate filament proteins, 17-β-hydroxysteroid dehydrogenase type 4, and kinase suppressor of Ras1. Western blot analysis of kinase suppressor of Ras1, glucose 6-phosphate dehydrogenase, elongation factor 2, 60 kDa heat-shock protein, and catalase supported the proteome evidence. Based on the changes found in the protein-expression levels of these proteins, we proposed a cellular-signalling pathway to explain the hepatic-cell response to the intake of a diet containing MA.<br /> (Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Analysis of Variance
Animal Feed
Animals
Blotting, Western
Electrophoresis, Gel, Two-Dimensional
Fish Proteins metabolism
Fisheries
Glucose metabolism
Metabolic Networks and Pathways drug effects
Nutrition Assessment
Peptide Fragments metabolism
Peptide Mapping
Proteome metabolism
Proteomics methods
Reproducibility of Results
Sea Bream growth & development
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Fish Proteins analysis
Liver metabolism
Proteome drug effects
Sea Bream metabolism
Triterpenes pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1615-9861
- Volume :
- 11
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 21751345
- Full Text :
- https://doi.org/10.1002/pmic.201000271