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Chlorogenic acid protects MSCs against oxidative stress by altering FOXO family genes and activating intrinsic pathway.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2012 Jan 15; Vol. 674 (2-3), pp. 65-72. Date of Electronic Publication: 2011 Jul 04. - Publication Year :
- 2012
-
Abstract
- Chlorogenic acid as an antioxidant exists widely in edible and medicinal plants, and can protect cell against apoptosis induced by oxidative stress. However, its molecular mechanisms remain largely unknown. Here, we showed that Chlorogenic acid suppressed reactive oxygen species increase by activation of Akt phosphorylation,and increased FOXO family genes and anti-apoptotic protein Bcl-2 expression in MSCs culturing under oxidative stress. In addition, PI-3Kinase Inhibitor (2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one, LY294002) could suppress the Chlorogenic acid-induced: (1) the cellular protective role, (2) the increase of the FOXO family genes expression, (3) increased expression of Bcl-2. These results suggested that Chlorogenic acid protected MSCs against apoptosis via PI3K/AKT signal and FOXO family genes.<br /> (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Apoptosis drug effects
Gene Expression Regulation drug effects
Hydrogen Peroxide pharmacology
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells metabolism
Phosphatidylinositol 3-Kinases metabolism
Phosphorylation drug effects
Proto-Oncogene Proteins c-akt metabolism
Proto-Oncogene Proteins c-bcl-2 metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species metabolism
Antioxidants pharmacology
Chlorogenic Acid pharmacology
Cytoprotection drug effects
Forkhead Transcription Factors genetics
Mesenchymal Stem Cells drug effects
Oxidative Stress drug effects
Signal Transduction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0712
- Volume :
- 674
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 21749866
- Full Text :
- https://doi.org/10.1016/j.ejphar.2011.06.033