Noninvasive fetal genotyping of human platelet antigen-1a.

Authors :: Scheffer PG
Ait Soussan A
Verhagen OJ
Page-Christiaens GC
Oepkes D
de Haas M
van der Schoot CE
Source :: BJOG : an international journal of obstetrics and gynaecology [BJOG] 2011 Oct; Vol. 118 (11), pp. 1392-5. Date of Electronic Publication: 2011 Jul 12.
Publication Year :: 2011
Abstract: We describe a reliable noninvasive fetal human platelet antigen (HPA)-1a genotyping assay on a real-time polymerase chain reaction (PCR) platform using cell-free fetal DNA isolated from maternal blood. Nonspecific amplification of maternal cell-free DNA is overcome by pre-PCR digestion of the cell-free DNA with the Msp1 restriction enzyme. Noninvasive fetal HPA-1a genotyping offers a safe method for alloimmunised pregnant women to determine whether their fetus is at risk of fetal or neonatal alloimmune thrombocytopenia (FNAIT) and whether interventions to prevent intracranial haemorrhage are required. The availability of this test is relevant to the ongoing debate on screening pregnancies for HPA-1a-mediated FNAIT.<br /> (© 2011 The Authors BJOG An International Journal of Obstetrics and Gynaecology © 2011 RCOG.)