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Evaluation of FKBP and DHFR based destabilizing domains in Saccharomyces cerevisiae.
- Source :
-
Bioorganic & medicinal chemistry letters [Bioorg Med Chem Lett] 2011 Sep 01; Vol. 21 (17), pp. 4965-8. Date of Electronic Publication: 2011 Jun 16. - Publication Year :
- 2011
-
Abstract
- Two orthogonal destabilizing domains have been developed based on mutants of human FKBP12 as well as bacterial DHFR and these engineered domains have been used to control protein concentration in a variety of contexts in vitro and in vivo. FKBP12 based destabilizing domains cannot be rescued in the yeast Saccharomyces cerevisiae; ecDHFR based destabilizing domains are not degraded as efficiently in S. cerevisiae as in mammalian cells or Plasmodium, but provide a starting point for the development of domains with increased signal-to-noise in S. cerevisiae.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Flow Cytometry
Humans
Mice
NIH 3T3 Cells
Saccharomyces cerevisiae growth & development
Tacrolimus Binding Protein 1A chemistry
Tetrahydrofolate Dehydrogenase chemistry
Saccharomyces cerevisiae metabolism
Tacrolimus Binding Protein 1A metabolism
Tetrahydrofolate Dehydrogenase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1464-3405
- Volume :
- 21
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Bioorganic & medicinal chemistry letters
- Publication Type :
- Academic Journal
- Accession number :
- 21741238
- Full Text :
- https://doi.org/10.1016/j.bmcl.2011.06.006