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Molecular expression and enzymatic characterization of thioredoxin from the carcinogenic human liver fluke Opisthorchis viverrini.
- Source :
-
Parasitology international [Parasitol Int] 2012 Mar; Vol. 61 (1), pp. 101-6. Date of Electronic Publication: 2011 Jun 29. - Publication Year :
- 2012
-
Abstract
- The human liver fluke, Opisthorchis viverrini, induces inflammation of the hepatobiliary system. Despite being constantly exposed to inimical oxygen radicals released from inflammatory cells, the parasite survives for years. Defense against oxidative damage can be mediated through glutathione and/or thioredoxin utilizing systems. Here, we report the molecular expression and biochemical characterization of a thioredoxin (Trx) from O. viverrini. O. viverrini Trx cDNA encoded a polypeptide of 105 amino acid residues, of molecular mass 11.63 kDa. The predicted protein has similarity to previously characterized thioredoxins with 26-51% identity. Recombinant O. viverrini Trx (Ov-Trx-1) was expressed as soluble protein in E. coli. The recombinant protein showed insulin reduction activity and supported the enzymatic function of O. viverrini thioredoxin peroxidase. Expression of Ov-Trx-1 at mRNA and protein levels was observed in all obtainable developmental stages of the liver fluke. Ov-Trx-1 was also detected in excretory-secretory products released by adult O. viverrini. Immunohistochemistry, Ov-Trx-1 was expressed in nearly all parasite tissue excepted ovary and mature sperms. Interestingly, Ov-Trx-1 was observed in the infected biliary epithelium but not in normal bile ducts. These results suggest that Ov-Trx-1 is essential for the parasite throughout the life cycle. In the host-parasite interaction aspect, Ov-Trx-1 may support thioredoxin peroxidase in protecting the parasite against damage induced by reactive oxygen species from inflammation.<br /> (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Bile Ducts, Intrahepatic metabolism
Bile Ducts, Intrahepatic parasitology
Blotting, Western
Cercaria chemistry
Cercaria growth & development
Chromatography, Affinity
DNA, Complementary genetics
Electrophoresis, Polyacrylamide Gel
Escherichia coli genetics
Gene Expression Profiling
Helminth Proteins chemistry
Mice
Opisthorchiasis enzymology
Opisthorchiasis genetics
Opisthorchis chemistry
Opisthorchis enzymology
Opisthorchis growth & development
Ovum chemistry
Ovum growth & development
Phylogeny
Recombinant Proteins chemistry
Recombinant Proteins genetics
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Thailand
Thioredoxins chemistry
Helminth Proteins metabolism
Opisthorchis genetics
Peroxiredoxins genetics
Thioredoxins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-0329
- Volume :
- 61
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Parasitology international
- Publication Type :
- Academic Journal
- Accession number :
- 21740981
- Full Text :
- https://doi.org/10.1016/j.parint.2011.06.018