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Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region.
- Source :
-
Molecular plant pathology [Mol Plant Pathol] 2011 Aug; Vol. 12 (6), pp. 535-47. Date of Electronic Publication: 2011 Jan 05. - Publication Year :
- 2011
-
Abstract
- Sharka disease, caused by the Plum pox virus (PPV), is one of the main limiting factors for stone fruit crops worldwide. Only a few resistance sources have been found in apricot (Prunus armeniaca L.), and most studies have located a major PPV resistance locus (PPVres) on linkage group 1 (LG1). However, the mapping accuracy was not sufficiently reliable and PPVres was predicted within a low confidence interval. In this study, we have constructed two high-density simple sequence repeat (SSR) improved maps with 0.70 and 0.68 markers/cm, corresponding to LG1 of 'Lito' and 'Goldrich' PPV-resistant cultivars, respectively. Using these maps, and excluding genotype-phenotype incongruent individuals, a new binary trait locus (BTL) analysis for PPV resistance was performed, narrowing down the PPVres support intervals to 7.3 and 5.9 cm in 'Lito' and 'Goldrich', respectively. Subsequently, 71 overlapping oligonucleotides (overgo) probes were hybridized against an apricot bacterial artificial chromosome (BAC) library, identifying 870 single BACs from which 340 were anchored onto a map region of approximately 30-40 cm encompassing PPVres. Partial BAC contigs assigned to the two allelic haplotypes (resistant/susceptible) of the PPVres locus were built by high-information content fingerprinting (HICF). In addition, a total of 300 BAC-derived sequences were obtained, and 257 showed significant homology with the peach genome scaffold&#95;1 corresponding to LG1. According to the peach syntenic genome sequence, PPVres was predicted within a region of 2.16 Mb in which a few candidate resistance genes were identified.<br /> (© 2011 The Authors. Molecular Plant Pathology © 2011 BSPP and Blackwell Publishing Ltd.)
- Subjects :
- Chromosome Mapping
Chromosome Segregation genetics
Chromosomes, Artificial, Bacterial genetics
Contig Mapping
Genes, Plant genetics
Genetic Association Studies
Genetic Loci genetics
Genetic Markers
Genotype
Microsatellite Repeats genetics
Plant Diseases genetics
Plant Diseases virology
Prunus immunology
Disease Resistance genetics
Genome, Plant genetics
Plant Diseases immunology
Plum Pox Virus immunology
Prunus genetics
Prunus virology
Synteny genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1364-3703
- Volume :
- 12
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Molecular plant pathology
- Publication Type :
- Academic Journal
- Accession number :
- 21722293
- Full Text :
- https://doi.org/10.1111/j.1364-3703.2010.00691.x