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The APC/C subunit 10 plays an essential role in cell proliferation during leaf development.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2011 Oct; Vol. 68 (2), pp. 351-63. Date of Electronic Publication: 2011 Aug 08. - Publication Year :
- 2011
-
Abstract
- The largest E3 ubiquitin-ligase complex, known as anaphase-promoting complex/cyclosome (APC/C), regulates the proteolysis of cell cycle regulators such as CYCLIN B and SECURIN that are essential for sister-chromatid separation and exit from mitosis. Despite its importance, the role of APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, the Arabidopsis thaliana APC/C subunit APC10 was characterized and shown to functionally complement an apc10 yeast mutant. The APC10 protein was located in specific nuclear bodies, most probably resulting from its association with the proteasome complex. An apc10 Arabidopsis knockout mutant strongly impaired female gametogenesis. Surprisingly, constitutive overexpression of APC10 enhanced leaf size. Through kinematic analysis, the increased leaf size was found to be due to enhanced rates of cell division during the early stages of leaf development and, at the molecular level, by increased APC/C activity as measured by an amplification of the proteolysis rate of the mitotic cyclin, CYCB1;1.<br /> (© 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.)
- Subjects :
- Arabidopsis genetics
Arabidopsis growth & development
Arabidopsis Proteins genetics
Biomechanical Phenomena
Cell Cycle Proteins genetics
Cell Cycle Proteins metabolism
Cyclin B genetics
Cyclin B metabolism
DNA, Complementary genetics
Gametogenesis, Plant genetics
Gene Expression Regulation, Plant genetics
Genetic Complementation Test
Genotype
Glucuronidase
Green Fluorescent Proteins
Mutation
Phenotype
Plant Leaves enzymology
Plant Leaves genetics
Plants, Genetically Modified
Proteolysis
RNA, Plant genetics
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Time Factors
Ubiquitin-Protein Ligases genetics
Arabidopsis enzymology
Arabidopsis Proteins metabolism
Cell Proliferation
Plant Leaves growth & development
Ubiquitin-Protein Ligases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1365-313X
- Volume :
- 68
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 21711400
- Full Text :
- https://doi.org/10.1111/j.1365-313X.2011.04691.x