Concentration and diversity of noroviruses detected in Luxembourg wastewaters in 2008-2009.

Authors :: Skraber S
Langlet J
Kremer JR
Mossong J
De Landtsheer S
Even J
Muller CP
Hoffmann L
Cauchie HM
Source :: Applied and environmental microbiology [Appl Environ Microbiol] 2011 Aug; Vol. 77 (15), pp. 5566-8. Date of Electronic Publication: 2011 Jun 24.
Publication Year :: 2011
Abstract: Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcription-PCR (RT-PCR), albeit at 30-fold lower concentrations than for genogroup II, hampering attempts to assess overall genetic diversity by the cloning/sequencing approach.

Subjects :: Base Sequence
Caliciviridae Infections genetics
Caliciviridae Infections virology
Genotype
Luxembourg
Molecular Sequence Data
Norovirus genetics
RNA, Viral analysis
RNA, Viral genetics
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, RNA
Norovirus isolation & purification
Sewage virology

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