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Altered Toll-like receptor 2-mediated endotoxin tolerance is related to diminished interferon beta production.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2011 Aug 26; Vol. 286 (34), pp. 29492-500. Date of Electronic Publication: 2011 Jun 24. - Publication Year :
- 2011
-
Abstract
- Induction of endotoxin tolerance leads to a reduced inflammatory response after repeated challenge by LPS and is important for resolution of inflammation and prevention of tissue damage. Enterobacterial LPS is recognized by the TLR4 signaling complex, whereas LPS of some non-enterobacterial organisms is capable of signaling independently of TLR4 utilizing TLR2-mediated signal transduction instead. In this study we report that Porphyromonas gingivalis LPS, a TLR2 agonist, fails to induce a fully endotoxin tolerant state in a human monocytic cell line (THP-1) and mouse bone marrow-derived macrophages. In contrast to significantly decreased production of human IL-8 and TNF-α and, in mice, keratinocyte-derived cytokine (KC), macrophage inflammatory protein-2 (MIP-2), and TNF-α after repeated challenge with Escherichia coli LPS, cells repeatedly exposed to P. gingivalis LPS responded by producing less TNF-α but sustained elevated secretion of IL-8, KC, and MIP-2. Furthermore, in endotoxin-tolerant cells, production of IL-8 is controlled at the signaling level and correlates well with NF-κB activation, whereas TNF-α expression is blocked at the gene transcription level. Interferon β plays an important role in attenuation of chemokine expression in endotoxin-tolerized cells as shown in interferon regulatory factor-3 knock-out mice. In addition, human gingival fibroblasts, commonly known not to display LPS tolerance, were found to be tolerant to repeated challenge by LPS if pretreated with interferon β. The data suggest that the inability of the LPS-TLR2 complex to induce full endotoxin tolerance in monocytes/macrophages is related to diminished production of interferon β and may partly explain the involvement of these LPS isoforms in the pathogenesis of chronic inflammatory diseases.
- Subjects :
- Animals
Cell Line
Cytokines biosynthesis
Cytokines genetics
Drug Resistance drug effects
Drug Resistance genetics
Humans
Interferon Regulatory Factor-3 genetics
Interferon Regulatory Factor-3 metabolism
Interferon-beta genetics
Mice
Mice, Knockout
NF-kappa B genetics
NF-kappa B metabolism
Signal Transduction drug effects
Signal Transduction genetics
Toll-Like Receptor 2 genetics
Fibroblasts metabolism
Interferon-beta biosynthesis
Lipopolysaccharides pharmacology
Macrophages metabolism
Toll-Like Receptor 2 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1083-351X
- Volume :
- 286
- Issue :
- 34
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 21705332
- Full Text :
- https://doi.org/10.1074/jbc.M111.252791