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Silencing activity of 2'-O-methyl modified anti-MDR1 siRNAs with mismatches in the central part of the duplexes.

Authors :
Petrova NS
Meschaninova MI
Venyaminova AG
Zenkova MA
Vlassov VV
Chernolovskaya EL
Source :
FEBS letters [FEBS Lett] 2011 Jul 21; Vol. 585 (14), pp. 2352-6. Date of Electronic Publication: 2011 Jun 23.
Publication Year :
2011

Abstract

The thermodynamic properties of siRNA duplexes are important for their silencing activity. siRNAs with high thermodynamic stability of both the central part of the duplex and in the whole, usually display low silencing activity. Destabilization of the central part of the siRNA duplex could increase its silencing activity. However, mismatches located in the central part of the duplex could substantially decrease the amount of RNAi efficacy, hindering active RISC formation and function. In this study, we examined the impact of duplex destabilization by nucleotide substitutions in the central part (7-10 nt counting from the 5'-end of the antisense strand) of the nuclease-resistant siRNA on its silencing activity.<br /> (Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-3468
Volume :
585
Issue :
14
Database :
MEDLINE
Journal :
FEBS letters
Publication Type :
Academic Journal
Accession number :
21704032
Full Text :
https://doi.org/10.1016/j.febslet.2011.06.003