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Silencing activity of 2'-O-methyl modified anti-MDR1 siRNAs with mismatches in the central part of the duplexes.
- Source :
-
FEBS letters [FEBS Lett] 2011 Jul 21; Vol. 585 (14), pp. 2352-6. Date of Electronic Publication: 2011 Jun 23. - Publication Year :
- 2011
-
Abstract
- The thermodynamic properties of siRNA duplexes are important for their silencing activity. siRNAs with high thermodynamic stability of both the central part of the duplex and in the whole, usually display low silencing activity. Destabilization of the central part of the siRNA duplex could increase its silencing activity. However, mismatches located in the central part of the duplex could substantially decrease the amount of RNAi efficacy, hindering active RISC formation and function. In this study, we examined the impact of duplex destabilization by nucleotide substitutions in the central part (7-10 nt counting from the 5'-end of the antisense strand) of the nuclease-resistant siRNA on its silencing activity.<br /> (Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- ATP Binding Cassette Transporter, Subfamily B
Animals
HEK293 Cells
Humans
Nucleotides genetics
RNA, Small Interfering chemistry
Thermodynamics
ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics
ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism
Base Pair Mismatch
Gene Silencing
RNA, Small Interfering genetics
RNA, Small Interfering metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 585
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 21704032
- Full Text :
- https://doi.org/10.1016/j.febslet.2011.06.003