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Detection of lumpy skin disease virus antigen and genomic DNA in formalin-fixed paraffin-embedded tissues from an Egyptian outbreak in 2006.
- Source :
-
Transboundary and emerging diseases [Transbound Emerg Dis] 2011 Oct; Vol. 58 (5), pp. 451-7. Date of Electronic Publication: 2011 Jun 23. - Publication Year :
- 2011
-
Abstract
- An outbreak of lumpy skin disease (LSD) was reported in 2006 in Egypt affecting 16 provinces. Biopsies and post-mortem tissue samples were collected from calves that showed typical clinical signs of LSD and fixed in formalin. These samples were collected from a private dairy farm in the Damietta province of Egypt. Formalin-fixed paraffin-embedded tissue samples were assessed using histology, and skin lesions were classified as either acute or subacute/chronic. Both lumpy skin disease virus (LSDV) DNA detected by polymerase chain reaction and LSDV antigen detected by immunohistochemistry using a capripoxvirus-specific monoclonal antibody were observed in the acute skin lesions and in some subacute/chronic skin lesions.<br /> (© 2011 Blackwell Verlag GmbH.)
- Subjects :
- Animals
Cattle
Dairying
Egypt epidemiology
Female
Formaldehyde
Lumpy Skin Disease epidemiology
Male
Time Factors
Tissue Preservation methods
Tissue Preservation veterinary
Antigens, Viral isolation & purification
DNA, Viral isolation & purification
Lumpy Skin Disease diagnosis
Lumpy skin disease virus genetics
Lumpy skin disease virus immunology
Paraffin Embedding veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1865-1682
- Volume :
- 58
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Transboundary and emerging diseases
- Publication Type :
- Academic Journal
- Accession number :
- 21699673
- Full Text :
- https://doi.org/10.1111/j.1865-1682.2011.01238.x