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Chlamydia pneumoniae infection induced allergic airway sensitization is controlled by regulatory T-cells and plasmacytoid dendritic cells.
- Source :
-
PloS one [PLoS One] 2011; Vol. 6 (6), pp. e20784. Date of Electronic Publication: 2011 Jun 10. - Publication Year :
- 2011
-
Abstract
- Chlamydia pneumoniae (CP) is associated with induction and exacerbation of asthma. CP infection can induce allergic airway sensitization in mice in a dose- and time-dependent manner. Allergen exposure 5 days after a low dose (mild-moderate), but not a high dose (severe) CP infection induces antigen sensitization in mice. Innate immune signals play a critical role in controlling CP infection induced allergic airway sensitization, however these mechanisms have not been fully elucidated. Wild-type, TLR2-/-, and TLR4-/- mice were infected intranasally (i.n.) with a low dose of CP, followed by i.n. exposure to human serum albumin (HSA) and challenged with HSA 2 weeks later. Airway inflammation, immunoglobulins, eosinophils, and goblet cells were measured. Low dose CP infection induced allergic sensitization in TLR2-/- mice, but not in TLR4-/- mice, due to differential Treg responses in these genotypes. TLR2-/- mice had reduced numbers of Tregs in the lung during CP infection while TLR4-/- mice had increased numbers. High dose CP infection resulted in an increase in Tregs and pDCs in lungs, which prevented antigen sensitization in WT mice. Depletion of Tregs or pDCs resulted in allergic airway sensitization. We conclude that Tregs and pDCs are critical determinants regulating CP infection-induced allergic sensitization. Furthermore, TLR2 and TLR4 signaling during CP infection may play a regulatory role through the modulation of Tregs.
- Subjects :
- Animals
Antigens, Bacterial immunology
Bordetella immunology
Bordetella radiation effects
Chlamydophila pneumoniae radiation effects
Eosinophils pathology
Humans
Inflammation complications
Inflammation microbiology
Inflammation pathology
Lung immunology
Lung microbiology
Lung pathology
Lymphocyte Depletion
Mice
Microbial Viability radiation effects
Pneumonia, Bacterial complications
Pneumonia, Bacterial pathology
Respiratory Hypersensitivity complications
Respiratory Hypersensitivity microbiology
Respiratory Hypersensitivity pathology
Serum Albumin immunology
Signal Transduction immunology
Signal Transduction radiation effects
Time Factors
Toll-Like Receptor 2 deficiency
Toll-Like Receptor 2 metabolism
Toll-Like Receptor 4 deficiency
Toll-Like Receptor 4 metabolism
Ultraviolet Rays
Chlamydophila pneumoniae immunology
Dendritic Cells immunology
Immunization
Pneumonia, Bacterial immunology
Pneumonia, Bacterial microbiology
Respiratory Hypersensitivity immunology
T-Lymphocytes, Regulatory immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 6
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 21695198
- Full Text :
- https://doi.org/10.1371/journal.pone.0020784