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Wound healing activity of the human antimicrobial peptide LL37.
- Source :
-
Peptides [Peptides] 2011 Jul; Vol. 32 (7), pp. 1469-76. Date of Electronic Publication: 2011 Jun 13. - Publication Year :
- 2011
-
Abstract
- Antimicrobial peptides (AMPs) are part of the innate immune system and are generally defined as cationic, amphipathic peptides, with less than 50 amino acids, including multiple arginine and lysine residues. The human cathelicidin antimicrobial peptide LL37 can be found at different concentrations in many different cells, tissues and body fluids and has a broad spectrum of antimicrobial and immunomodulatory activities. The healing of wound is a complex process that involves different steps: hemostasis, inflammation, remodeling/granulation tissue formation and re-epithelialization. Inflammation and angiogenesis are two fundamental physiological conditions implicated in this process. We have recently developed a new method for the expression and purification of recombinant LL37. In this work, we show that the recombinant peptide P-LL37 with a N-terminus proline preserves its immunophysiological properties in vitro and in vivo. P-LL37 neutralized the activation of macrophages by lipopolysaccharide (LPS). Besides, the peptide induced proliferation, migration and tubule-like structures formation by endothelial cells. Wound healing experiments were performed in dexamethasone-treated mice to study the effect of LL37 on angiogenesis and wound regeneration. The topical application of synthetic and recombinant LL37 increased vascularization and re-epithelialization. Taken together, these results clearly demonstrate that LL37 may have a key role in wound regeneration through vascularization.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Anti-Bacterial Agents chemistry
Antimicrobial Cationic Peptides
Cathelicidins chemistry
Cell Movement drug effects
Cell Survival drug effects
Dexamethasone adverse effects
Dexamethasone pharmacology
Endothelial Cells cytology
Endothelium, Vascular cytology
Endothelium, Vascular drug effects
Granulation Tissue cytology
Granulation Tissue drug effects
Granulation Tissue physiology
Humans
Lipopolysaccharides antagonists & inhibitors
Lipopolysaccharides pharmacology
Macrophages cytology
Macrophages drug effects
Mice
Mice, Inbred C57BL
Microscopy
Recombinant Proteins chemistry
Soft Tissue Injuries immunology
Soft Tissue Injuries pathology
Wound Healing physiology
Anti-Bacterial Agents pharmacology
Cathelicidins pharmacology
Endothelial Cells drug effects
Neovascularization, Physiologic drug effects
Recombinant Proteins pharmacology
Soft Tissue Injuries drug therapy
Wound Healing drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1873-5169
- Volume :
- 32
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Peptides
- Publication Type :
- Academic Journal
- Accession number :
- 21693141
- Full Text :
- https://doi.org/10.1016/j.peptides.2011.06.005