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Responses to water stress of gas exchange and metabolites in Eucalyptus and Acacia spp.
- Source :
-
Plant, cell & environment [Plant Cell Environ] 2011 Oct; Vol. 34 (10), pp. 1609-29. Date of Electronic Publication: 2011 Jun 22. - Publication Year :
- 2011
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Abstract
- Studies of water stress commonly examine either gas exchange or leaf metabolites, and many fail to quantify the concentration of CO₂ in the chloroplasts (C(c)). We redress these limitations by quantifying C(c) from discrimination against ¹³CO₂ and using gas chromatography-mass spectrometry (GC-MS) for leaf metabolite profiling. Five Eucalyptus and two Acacia species from semi-arid to mesic habitats were subjected to a 2 month water stress treatment (Ψ(pre-dawn) = -1.7 to -2.3 MPa). Carbohydrates dominated the leaf metabolite profiles of species from dry areas, whereas organic acids dominated the metabolite profiles of species from wet areas. Water stress caused large decreases in photosynthesis and C(c), increases in 17-33 metabolites and decreases in 0-9 metabolites. In most species, fructose, glucose and sucrose made major contributions to osmotic adjustment. In Acacia, significant osmotic adjustment was also caused by increases in pinitol, pipecolic acid and trans-4-hydroxypipecolic acid. There were also increases in low-abundance metabolites (e.g. proline and erythritol), and metabolites that are indicative of stress-induced changes in metabolism [e.g. γ-aminobutyric acid (GABA) shunt, photorespiration, phenylpropanoid pathway]. The response of gas exchange to water stress and rewatering is rather consistent among species originating from mesic to semi-arid habitats, and the general response of metabolites to water stress is rather similar, although the specific metabolites involved may vary.<br /> (© 2011 Blackwell Publishing Ltd.)
- Subjects :
- Acacia metabolism
Australia
Carbon Dioxide metabolism
Carbon Isotopes analysis
Chloroplasts metabolism
Dehydration
Ecosystem
Eucalyptus chemistry
Eucalyptus metabolism
Osmosis
Plant Epidermis metabolism
Plant Leaves chemistry
Plant Leaves metabolism
Plant Stomata metabolism
Water metabolism
Acacia physiology
Eucalyptus physiology
Metabolome physiology
Photosynthesis physiology
Plant Transpiration physiology
Stress, Physiological physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-3040
- Volume :
- 34
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Plant, cell & environment
- Publication Type :
- Academic Journal
- Accession number :
- 21692813
- Full Text :
- https://doi.org/10.1111/j.1365-3040.2011.02357.x