Protocol for high-resolution electrophoresis separation of myosin heavy chain isoforms in bovine skeletal muscle.

Authors :: Picard B
Barboiron C
Chadeyron D
Jurie C
Source :: Electrophoresis [Electrophoresis] 2011 Jul; Vol. 32 (14), pp. 1804-6. Date of Electronic Publication: 2011 Jun 20.
Publication Year :: 2011
Abstract: In this short communication we describe a specific protocol for SDS-PAGE separation of adult bovine myosin heavy-chain (MyHC) isoforms. The conditions defined in this protocol allow a good separation with a good reproducibility of the four MyHC isoforms (MyHC I, IIa, IIx, IIb) identified in adult skeletal muscle of this species. This procedure uses mini-gel electrophoresis system and does not involve preparation of gradient separating gels. In addition, this protocol can also be applied to the electrophoretic separation of ovine and camel MyHC isoforms.<br /> (Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: Animals
Camelus
Cattle
Humans
Myosin Heavy Chains chemistry
Protein Isoforms
Rats
Reproducibility of Results
Sheep
Skeletal Muscle Myosins chemistry
Electrophoresis, Polyacrylamide Gel methods
Muscle, Skeletal chemistry
Myosin Heavy Chains isolation & purification
Skeletal Muscle Myosins isolation & purification

Full Text Access

Tools