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Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts.
- Source :
-
International journal of molecular medicine [Int J Mol Med] 2011 Sep; Vol. 28 (3), pp. 315-25. Date of Electronic Publication: 2011 Jun 08. - Publication Year :
- 2011
-
Abstract
- The aim of the present study was to investigate the influence of a decellularization protocol on the structure and the mechanical behavior of small-diameter (<6 mm) tibial calf arteries and veins. Calf vessels were decellularized by a detergent-enzymatic method (DEM), partially hydrolyzed with trypsin and subsequently cross-linked using poly(ethylene glycol) diglycidyl ether. Our results showed that i) the DEM can be considered a simple and valuable procedure for the preparation of complete acellular arteries and veins able to preserve a high degree of collagen and elastic fibers, and ii) poly(ethylene glycol) diglycidyl ether cross-linking treatment provides appropriate mechanical reinforcement of blood vessels. Histologically, the decellularized vessels were obtained employing the detergent-enzymatic procedure and their native extracellular matrix histoarchitecture and components remained well preserved. Moreover, the decellularization protocol can be considered an effective method to remove HLA class I antigen expression from small-diameter tibial calf arteries and veins. Cytocompatibility of decellularized cross-linked vessels was evaluated by endothelial and smooth muscle cell seeding on luminal and adventitial vessel surfaces, respectively.
- Subjects :
- Animals
Blood Vessels cytology
Cattle
Cell Adhesion
Cell Proliferation
Cells, Cultured
Collagen metabolism
Cross-Linking Reagents metabolism
Endothelial Cells cytology
Epoxy Resins metabolism
Glycine metabolism
Humans
Immunohistochemistry
Microscopy, Electron, Scanning
Myocytes, Smooth Muscle cytology
Trypsin metabolism
Blood Vessel Prosthesis
Blood Vessels transplantation
Tissue Engineering methods
Subjects
Details
- Language :
- English
- ISSN :
- 1791-244X
- Volume :
- 28
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 21667016
- Full Text :
- https://doi.org/10.3892/ijmm.2011.720