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Interplay of early biochemical manifestations by cadmium insult in sertoli-germ coculture: an in vitro study.
- Source :
-
Toxicology [Toxicology] 2011 Sep 05; Vol. 287 (1-3), pp. 46-53. Date of Electronic Publication: 2011 May 30. - Publication Year :
- 2011
-
Abstract
- Cadmium is a common environmental and occupational hazard and its adverse effect on reproductive organ has been well documented. The present study is planned to delineate the mechanism of Cd toxicity in rat testes. Our study shows that Cd causes apoptosis in sertoli-germ cells which is governed by oxidative stress. We assayed ROS, GSH and MMP to ensure the role of oxidative stress, further confirmed it by thiol modulators. The initial biochemical response shown in sertoli-germ cells was a significant rise in intracellular calcium followed by a drastic fall in MMP and then ROS generation. The downstream events included cytochrome c release leading to caspase-3 activation and culminating in cell death via apoptosis. Furthermore Cd disrupted the spermatogenic pathway as evident by suppression in tesmin and LDH-X levels.<br /> (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Animals
Caspase 3 metabolism
Cell Survival drug effects
Coculture Techniques
Cytochromes c metabolism
DNA Damage
Lipid Peroxidation drug effects
Male
Membrane Potential, Mitochondrial drug effects
Rats
Rats, Wistar
Reactive Oxygen Species metabolism
Sertoli Cells metabolism
Spermatozoa metabolism
Cadmium toxicity
Sertoli Cells drug effects
Spermatozoa drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3185
- Volume :
- 287
- Issue :
- 1-3
- Database :
- MEDLINE
- Journal :
- Toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 21664405
- Full Text :
- https://doi.org/10.1016/j.tox.2011.05.013