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Beta3-adrenoceptors in the rat sacral spinal cord and their functional relevance in micturition under normal conditions and in a model of partial urethral obstruction.
- Source :
-
Neurourology and urodynamics [Neurourol Urodyn] 2011 Sep; Vol. 30 (7), pp. 1382-7. Date of Electronic Publication: 2011 Jun 09. - Publication Year :
- 2011
-
Abstract
- Aims: Beta3-adrenoceptor selective agonists are evaluated as a new treatment for patients with lower urinary tract symptoms . It is believed that β3-AR selective agonists exert their effects via a peripheral site of action. However, β3-ARs have been found in brain tissue. This study examined whether β3-ARs are present in rat sacral spinal cord, and whether there are differences in β3-AR expression between normal and partial urethral obstruction (PUO) animals, and furthermore assessed the functional relevance of spinal β3-ARs for micturition.<br />Methods: Thirty-eight male Sprague-Dawley rats underwent either PUO or sham-operation. Two weeks after operation, half of the animals were used for histomorphological analysis. Remaining animals were used for functional experiments, where a β3-AR selective agonist, BRL 37344, was given intrathecally. Bladder function was assessed by continuous cystometry in non-anesthetized animals before and after drug administration.<br />Results: Beta3-ARs were found in sacral spinal cord segments with an accumulation in the ventral horn. There was a significant increase of β3-AR expression in obstructed rats. In functional experiments obstructed rats showed increased bladder weight, micturition frequency, spontaneous activity, and bladder pressures (all P < 0.05) compared to controls. Intrathecally administered BRL 37344 showed no effect in non-obstructed rats. In obstructed rats intrathecal BRL 37344 significantly reduced bladder pressures, spontaneous activity, and micturition frequency (all P < 0.05).<br />Conclusions: Beta3-ARs are present in rat sacral spinal cord, and are significantly up-regulated after PUO. Besides their well-established peripheral site of action in the treatment of voiding dysfunction, β3-AR selective agonists might exert relevant effects at a central nervous site of action.<br /> (Copyright © 2011 Wiley-Liss, Inc.)
- Subjects :
- Adrenergic beta-3 Receptor Agonists administration & dosage
Animals
Blotting, Western
Disease Models, Animal
Ethanolamines administration & dosage
Immunohistochemistry
Male
Pilot Projects
Rats
Rats, Sprague-Dawley
Sacrum
Spinal Cord drug effects
Spinal Cord physiopathology
Time Factors
Up-Regulation
Urethral Obstruction drug therapy
Urethral Obstruction physiopathology
Urodynamics
Receptors, Adrenergic, beta-3 metabolism
Spinal Cord metabolism
Urethral Obstruction metabolism
Urinary Bladder innervation
Urination drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6777
- Volume :
- 30
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Neurourology and urodynamics
- Publication Type :
- Academic Journal
- Accession number :
- 21661032
- Full Text :
- https://doi.org/10.1002/nau.21071