Back to Search
Start Over
Possible origin of CSF antibodies induced by intrathecal immunization and prophylactic effects against intracerebral rabies virus infection.
- Source :
-
The Journal of veterinary medical science [J Vet Med Sci] 2011 Oct; Vol. 73 (10), pp. 1303-8. Date of Electronic Publication: 2011 Jun 07. - Publication Year :
- 2011
-
Abstract
- Intrathecal (IT) immunization involves injecting antigens directly into the intraventricular or subarachnoid spaces, or brain, to induce antigen-specific antibodies (Ab) in the cerebrospinal fluid (CSF). In the present study, rabbits were immunized IT with inactivated rabies virus to investigate the origins of CSF Ab. The time course of Ab induction and tumor necrosis factor-alpha expression suggested the possibility that the CSF Ab originated in the serum. In addition, Ab-producing cells infiltrated around the blood vessels of the brain, suggesting local production of Ab within the central nervous system (CNS). Furthermore, subcutaneous (SC) immunization prior to IT immunization induced a rapid and magnified Ab response in the CSF compared with IT immunization alone. These results were confirmed by the fact that mice immunized SC prior to IT were more resistant to intracerebral challenge with rabies virus than mice immunized via the IT route alone. Taken together, these results suggest that combined SC and IT immunization is a more effective vaccination protocol for prophylaxis and treatment of rabies.
- Subjects :
- Animals
Antibodies, Viral blood
Enzyme-Linked Immunosorbent Assay
Female
Injections, Spinal
Injections, Subcutaneous
Mice
Mice, Inbred ICR
Rabbits
Rabies metabolism
Rabies prevention & control
Rabies Vaccines administration & dosage
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha metabolism
Antibodies, Viral cerebrospinal fluid
Rabies immunology
Rabies Vaccines immunology
Rabies virus immunology
Vaccination methods
Subjects
Details
- Language :
- English
- ISSN :
- 1347-7439
- Volume :
- 73
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- The Journal of veterinary medical science
- Publication Type :
- Academic Journal
- Accession number :
- 21646749
- Full Text :
- https://doi.org/10.1292/jvms.11-0183