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Selective chemiluminescence method for monitoring of vitamin K homologues in rheumatoid arthritis patients.

Authors :
Ahmed S
Kishikawa N
Ohyama K
Imazato T
Ueki Y
Kuroda N
Source :
Talanta [Talanta] 2011 Jul 15; Vol. 85 (1), pp. 230-6. Date of Electronic Publication: 2011 Apr 13.
Publication Year :
2011

Abstract

Vitamin K is a fat-soluble vitamin involved in blood coagulation and bone metabolism. The detection and monitoring of vitamin K homologues in rheumatoid arthritis (RA) patients is a challenging problem due to the smaller concentrations of vitamin K and the presence of several interfering medications. Therefore, this study aimed to develop a new highly sensitive and selective chemiluminescence (CL) method designated to quantify vitamin K homologues in plasma of RA patients including phylloquinone (PK, vitamin K(1)), menaquinone-4 (MK-4, vitamin K(2)) and menaquinone-7 (MK-7, vitamin K(2)). The method was based on the unique photochemical properties of vitamin K homologues that were exploited for selective luminol CL reaction. The correlation coefficients of 0.998 or more were obtained in the concentration ranges of 0.1-100 ng mL(-1) vitamin K homologues. The detection limits were 0.03-0.1 ng mL(-1) in human plasma for vitamin K homologues. The developed HPLC-CL system was successfully applied for selective determination of vitamin K homologues in plasma of RA patients. The developed method may provide a useful tool for monitoring vitamin K homologues in different clinical studies such as RA, osteoporosis and hepatocellular carcinoma in which vitamin K is intervented.<br /> (Copyright © 2011 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-3573
Volume :
85
Issue :
1
Database :
MEDLINE
Journal :
Talanta
Publication Type :
Academic Journal
Accession number :
21645693
Full Text :
https://doi.org/10.1016/j.talanta.2011.03.059