Back to Search
Start Over
Optical detection of chloramphenicol using molecularly imprinted polymers.
- Source :
-
Analytical chemistry [Anal Chem] 1997 Jun 01; Vol. 69 (11), pp. 2017-21. - Publication Year :
- 1997
-
Abstract
- A practical optical sensing system for the determination of chloramphenicol (CAP), utilizing molecularly imprinted polymers (MIPs) and HPLC, has been developed. The method is based on competitive displacement of a chloramphenicol-methyl red (CAP-MR) dye conjugate from specific binding cavities in an imprinted polymer by the analyte. The best of these polymers was obtained using (diethylamino)ethyl methacrylate as functional monomer at a monomer:template ratio of 2:1. HPLC with a mobile phase containing CAP-MR was used as the detection system, and injection of CAP and, to a lesser degree, thiamphenicol resulted in proportional displacement of the conjugate, which was detected at 460 nm. The detection system showed a linear response over a range of 3-1000 μg/mL and effectively detected CAP extracted from serum. This system offers a tailor-made, selective, and rapid method for CAP detection, is able to discriminate between similar molecules, and is effective below and above the therapeutic range (10-20 μg/mL serum, potentially toxic above 25 μg/mL). This technique is quite general and should enable the use of MIPs in a wide variety of applications involving the detection of families of molecules which possess a distinct arrangement of functional groups.
Details
- Language :
- English
- ISSN :
- 0003-2700
- Volume :
- 69
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 21639240
- Full Text :
- https://doi.org/10.1021/ac960983b