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Simultaneous determination of trace sterols in complicated biological samples by gas chromatography-mass spectrometry coupled with extraction using β-sitosterol magnetic molecularly imprinted polymer beads.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2011 Jul 15; Vol. 1218 (28), pp. 4275-83. Date of Electronic Publication: 2011 May 14. - Publication Year :
- 2011
-
Abstract
- In this paper, an efficient and sensitive analytical method for the simultaneous determination of three trace sterols including ergosterol, stigmasterol and β-sitosterol in complicated biological samples was developed by gas chromatography-mass spectrometry (GC-MS) coupled with extraction using novel β-sitosterol magnetic molecularly imprinted polymer (mag-MIP) beads. Physical tests suggested that β-sitosterol mag-MIP beads prepared by a rapid microwave synthesis method possessed the porous morphology, narrow size distribution, stable chemical and thermal property. Due to the greatly enlarging surface area and the strong recognition to the target molecules, β-sitosterol mag-MIP beads have a higher enrichment factor for β-sitosterol (∼20-fold) and the higher selectivity for β-sitosterol and its analogs than that of β-sitosterol magnetic nonimprinted polymer (mag-NIP) beads. Under the optimum analytical conditions, all the target compounds achieved good chromatographic separation and sensitive detection without matrix interference. It was interesting that three target sterols were actually found in mushroom samples, and stigmasterol and β-sitosterol were actually found in serum and watermelon samples. The recoveries of spiked sample tests were in range of 71.6-88.2% with RSDs of 2.4-10.0% (n=3). This method is reliable and applicable for the simultaneous determination of trace sterols in real biological samples based on the β-sitosterol mag-MIP bead extraction.<br /> (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Subjects :
- Agaricales chemistry
Citrullus chemistry
Humans
Microscopy, Electron, Scanning
Microspheres
Particle Size
Reproducibility of Results
Sensitivity and Specificity
Sitosterols chemistry
Sitosterols isolation & purification
Sterols blood
Sterols isolation & purification
Chemical Fractionation methods
Gas Chromatography-Mass Spectrometry methods
Molecular Imprinting methods
Sitosterols analysis
Sterols analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3778
- Volume :
- 1218
- Issue :
- 28
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 21632061
- Full Text :
- https://doi.org/10.1016/j.chroma.2011.05.022