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Diagnostic accuracy of using erythrocyte indices and polychromasia to identify regenerative anemia in dogs.
- Source :
-
Journal of the American Veterinary Medical Association [J Am Vet Med Assoc] 2011 Jun 01; Vol. 238 (11), pp. 1452-8. - Publication Year :
- 2011
-
Abstract
- Objective: To determine diagnostic accuracy of using erythrocyte indices and polychromasia to identify regenerative anemia in dogs.<br />Design: Retrospective and prospective cross-sectional study.<br />Animals: 4,521 anemic dogs.<br />Procedures: CBC results obtained between July 2002 and July 2008 by use of an automated laser-based flow cytometric hematology analyzer from dogs with Hct values ≤ 35% were retrieved. Sensitivity, specificity, accuracy, and predictive values of using erythrocyte indices and polychromasia to identify regeneration were determined, with a reticulocyte count > 65,000 reticulocytes/μL considered the gold standard. Similarly, 134 blood samples from anemic dogs were analyzed prospectively with an in-house electrical impedance analyzer.<br />Results: Of 4,387 dogs with samples analyzed retrospectively, 1,426 (32.5%) had regenerative anemia. Of these, 168 (11.8%) had macrocytic hypochromic anemia. High mean cell volume and low mean cell hemoglobin concentration had low sensitivity (11%), high specificity (98%), and moderate accuracy (70%) when used to identify regenerative anemia. Use of polychromasia alone had an accuracy of 77%, and use of polychromasia combined with a high RBC distribution width (RDW) had an accuracy of 79%. Results obtained with the in-house analyzer were similar.<br />Conclusions and Clinical Relevance: Results suggested that most regenerative anemias in dogs were not macrocytic hypochromic. Polychromasia, with or without high RDW, was a more accurate indicator than other erythrocyte indices of regenerative anemia. To avoid a false diagnosis of nonregenerative anemia, a blood smear should be evaluated in anemic dogs when a reticulocyte count is not available.
- Subjects :
- Anemia blood
Anemia diagnosis
Animals
Blood Cell Count veterinary
Cross-Sectional Studies
Diagnosis, Differential
Dogs
Erythrocyte Count veterinary
Erythrocyte Indices veterinary
Female
Hematocrit veterinary
Hematologic Diseases blood
Hematologic Diseases diagnosis
Hematologic Diseases veterinary
Male
Prospective Studies
Reproducibility of Results
Reticulocyte Count veterinary
Retrospective Studies
Sensitivity and Specificity
Anemia veterinary
Dog Diseases blood
Dog Diseases diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1943-569X
- Volume :
- 238
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of the American Veterinary Medical Association
- Publication Type :
- Academic Journal
- Accession number :
- 21627508
- Full Text :
- https://doi.org/10.2460/javma.238.11.1452