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Identification of CD8+ cytotoxic T lymphocyte epitopes from porcine reproductive and respiratory syndrome virus matrix protein in BALB/c mice.
- Source :
-
Virology journal [Virol J] 2011 May 30; Vol. 8, pp. 263. Date of Electronic Publication: 2011 May 30. - Publication Year :
- 2011
-
Abstract
- Twenty-seven nanopeptides derived from the matrix (M) protein of porcine reproductive and respiratory syndrome virus (PRRSV) were screened for their ability to elicit a recall interferon-γ (IFN-γ) response from the splenocytes of BALB/c mice following DNA vaccination and a booster vaccination with recombinant vaccinia virus rWR-PRRSV-M. We identified two peptides (amino acid residues K93FITSRCRL and F57GYMTFVHF) as CD8+ cytotoxic T lymphocyte (CTL) epitopes. These peptides elicited significant numbers of IFN-γ secreting cells, compared with other M nonapeptides and one irrelevant nonapeptide. Bioinformatics analysis showed that the former is an H-2Kd-restricted CTL epitope, and the latter is an H-2Dd-restricted CTL epitope. Multiple amino acid sequence alignment among different PRRSV M sequences submitted to GenBank indicated that these two CTL epitopes are strongly conserved, and they should therefore be considered for further research on the mechanisms of cellular immune responses to PRRSV.
- Subjects :
- Animals
Drug Carriers administration & dosage
Female
Genetic Vectors
Interferon-gamma metabolism
Mice
Mice, Inbred BALB C
Vaccination methods
Vaccinia virus genetics
Viral Vaccines administration & dosage
Viral Vaccines immunology
Epitope Mapping
Epitopes, T-Lymphocyte immunology
Porcine respiratory and reproductive syndrome virus immunology
T-Lymphocytes, Cytotoxic immunology
Viral Matrix Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1743-422X
- Volume :
- 8
- Database :
- MEDLINE
- Journal :
- Virology journal
- Publication Type :
- Academic Journal
- Accession number :
- 21619712
- Full Text :
- https://doi.org/10.1186/1743-422X-8-263