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Altered regulation of renal nitric oxide and atrial natriuretic peptide systems in angiotensin II-induced hypertension.
- Source :
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Regulatory peptides [Regul Pept] 2011 Oct 10; Vol. 170 (1-3), pp. 31-7. Date of Electronic Publication: 2011 May 25. - Publication Year :
- 2011
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Abstract
- The present study was aimed to determine whether there is an altered role of local nitric oxide (NO) and atrial natriuretic peptide (ANP) systems in the kidney in association with the angiotensin (Ang) II-induced hypertension. Male Sprague-Dawley rats were used. Ang II (100 ng·min⁻¹·kg⁻¹) was infused through entire time course. Thirteenth day after beginning the regimen, kidneys were taken. The protein expression of NO synthase (NOS) and nitrotyrosine was determined by semiquantitative immunoblotting. The mRNA expression of components of ANP system was determined by real-time polymerase chain reaction. The activities of soluble and particulate guanylyl cyclases were determined by the amount of cGMP generated in responses to sodium nitroprusside and ANP, respectively. There developed hypertension and decreased creatinine clearance in the experimental group. The protein expression of eNOS, nNOS and nitrotyrosine was increased in the cortex, while that of iNOS remained unaltered. The urinary excretion of NO increased in Ang II-induced hypertensive rats. The catalytic activity of soluble guanylyl cyclase was blunted in the glomerulus in Ang II-induced hypertensive rats. The mRNA expression of ANP was increased in Ang II-induced hypertensive rats. Neither the expression of NPR-A nor that of NPR-C was changed. The protein expression of neutral endopeptidase was decreased and the activity of particulate guanylyl cyclase was blunted in the glomerulus and papilla in Ang II-induced hypertensive rats. In conclusion, the synthesis of NO and ANP was increased in the kidney of Ang II-induced hypertension, while stimulated cGMP response was blunted. These results suggest desensitization of guanylyl cyclase in the kidney of Ang II-induced hypertensive rats, which may contribute to the associated renal vasoconstriction and hypertension.<br /> (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Subjects :
- Angiotensin II
Animals
Atrial Natriuretic Factor genetics
Blood Pressure
Cyclic GMP metabolism
Hypertension metabolism
Hypertension physiopathology
Kidney enzymology
Kidney physiopathology
Kidney Function Tests
Male
Neprilysin metabolism
Nitric Oxide urine
Nitric Oxide Synthase metabolism
Rats
Rats, Sprague-Dawley
Transcription, Genetic
Tyrosine analogs & derivatives
Tyrosine metabolism
Atrial Natriuretic Factor metabolism
Hypertension chemically induced
Kidney metabolism
Nitric Oxide metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-1686
- Volume :
- 170
- Issue :
- 1-3
- Database :
- MEDLINE
- Journal :
- Regulatory peptides
- Publication Type :
- Academic Journal
- Accession number :
- 21616096
- Full Text :
- https://doi.org/10.1016/j.regpep.2011.05.005