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Photoprotecting action and phytochemical analysis of a multiple radical scavenger lipophilic fraction obtained from the leaf of the seagrass Thalassia testudinum.
- Source :
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Photochemistry and photobiology [Photochem Photobiol] 2011 Sep-Oct; Vol. 87 (5), pp. 1058-66. Date of Electronic Publication: 2011 Jul 07. - Publication Year :
- 2011
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Abstract
- The apolar fraction F1 of Thalassia testudinum was chemically characterized by gas chromatography-mass spectrometry, which led to the identification of 43 metabolites, all of them reported for the first time in the genus Thalassia. More than 80% of the F1 composition was constituted by aromatic metabolites including the major components 1,1-bis(p-tolyl)ethane (6.0%), 4,4'-diisopropylbiphenyl (4.8%) and a 1,1-bis(p-tolyl)ethane isomer (4.7%). This lipophilic fraction was assayed for its antioxidant effects and skin protective action. In vitro assays showed that F1 strongly scavenged DPPH* (IC(50) 312.0 ± 8.0 μg mL(-1)), hydroxyl (IC(50) 23.8 ± 0.5 μg mL(-1)) and peroxyl radical (IC(50) 6.6 ± 0.3 μg mL(-1) ), as well as superoxide anion (IC(50) 50.0 ± 0.7 μg mL(-1)). Also, F1 markedly inhibited the spontaneous lipid peroxidation (LPO) in brain homogenates (IC(50) 93.0 ± 6.0 μg mL(-1)) and the LPS-stimulated nitrite generation on RAW624.7 macrophages (58.6 ± 3.2%, 400 μg mL(-1)). In agreement with these findings, its topical application at 250 and 500 μg cm(-2) strikingly reduced skin damage on mice exposed to acute UVB radiation by 45% and 70%, respectively and significantly attenuated the LPO developed following the first 48 h after acute exposure to UVB irradiation, as manifested by the decreased malondialdehide level and by the increased of reduced gluthatione content. Our results suggest that F1 may contribute to skin repair by attenuating oxidative stress due to its antioxidant activity.<br /> (© 2011 The Authors. Photochemistry and Photobiology © 2011 The American Society of Photobiology.)
- Subjects :
- Animals
Brain drug effects
Brain metabolism
Cell Line
Free Radical Scavengers chemistry
Free Radical Scavengers metabolism
Free Radicals metabolism
Gas Chromatography-Mass Spectrometry
Glutathione analysis
Glutathione biosynthesis
Hydrophobic and Hydrophilic Interactions
Lipid Peroxidation drug effects
Lipid Peroxidation radiation effects
Lipopolysaccharides adverse effects
Macrophages cytology
Macrophages drug effects
Macrophages metabolism
Male
Malondialdehyde analysis
Mice
Plant Extracts chemistry
Plant Extracts metabolism
Skin metabolism
Skin radiation effects
Sunscreening Agents chemistry
Sunscreening Agents metabolism
Tissue Extracts metabolism
Ultraviolet Rays adverse effects
Free Radical Scavengers pharmacology
Free Radicals antagonists & inhibitors
Hydrocharitaceae chemistry
Plant Extracts pharmacology
Plant Leaves chemistry
Skin drug effects
Sunscreening Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1751-1097
- Volume :
- 87
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Photochemistry and photobiology
- Publication Type :
- Academic Journal
- Accession number :
- 21615743
- Full Text :
- https://doi.org/10.1111/j.1751-1097.2011.00945.x