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Correlation between Fc γ R III a and aortic atherosclerotic plaque destabilization in ApoE knockout mice and intervention effects of effective components of chuanxiong rhizome and red peony root.
- Source :
-
Chinese journal of integrative medicine [Chin J Integr Med] 2011 May; Vol. 17 (5), pp. 355-60. Date of Electronic Publication: 2011 May 25. - Publication Year :
- 2011
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Abstract
- Objective: To explore the correlation between Fc γ RIII A (CD16A) and aortic atherosclerotic plaque destabilization in apoE knockout (apoE KO) mice and the intervention effects of effective components of chuanxiong rhizome and red peony root.<br />Methods: Eight 8-week-old male C57BL/6J mice were selected as the control group. Forty 8-week-old male apoE KO mice were randomly divided into the model group, apoE KO + intraperitoneal injection immunoglobulin group (IVIG), apoE KO + simvastatin group (Sm), apoE KO + high dosage of xiongshao capsule (XSC) group (XSCH), and apoE KO + low dosage of XSC group (XSCL), 8 mice in each group. Mice in the control group were put on a normal diet, and others were fed with a high-fat diet. After 10-week different interventions, monocyte CD16 expression was detected by flow cytometry, aortic matrix metalloproteinase-9 (MMP-9) mRNA expression was detected using reverse transcription polymerase chain reaction, and serum tumor necrosis factor (TNF)-α level was detected using enzyme-linked immunosorbent assay.<br />Results: Compared with the control group, monocyte CD16 expression, aortic MMP-9 mRNA expression, and serum TNF-α level in the model group increased obviously (P<0.01). Injections of apoE KO mice with intraperitoneal immunoglobulin during a 5-day period significantly reduced the monocyte CD16 expression, aortic MMP-9 mRNA expression, and serum TNF-α level (P<0.01 or 0.05) over a 10-week period of high-fat diet. Indices above in the Sm group, XSCH group, and XSCL group decreased in a different degree. Of them, the aortic MMP-9 mRNA expression in XSCH group was lower than that in Sm group (P<0.05) and the monocyte CD16 expression and serum TNF-α level showed no significant difference between XSCH group and Sm group (P>0.05). Correlation analyses suggested positive correlation between monocyte CD16 expression and aortic MMP-9 mRNA expression or serum TNF-α level in IVIG group, XSCH group, and XSCL group.<br />Conclusions: FcγR III A mediates systemic inflammation in the progression of coronary heart disease with blood stasis syndrome. XSC could stabilize atherosclerotic plaque by suppressing inflammation and its target was relative with FcγRIII A.
- Subjects :
- Animals
Aorta drug effects
Aorta enzymology
Apolipoproteins E metabolism
Drugs, Chinese Herbal pharmacology
Flow Cytometry
Gene Expression Regulation, Enzymologic drug effects
Lipopolysaccharide Receptors metabolism
Male
Matrix Metalloproteinase 9 genetics
Matrix Metalloproteinase 9 metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Monocytes drug effects
Monocytes metabolism
Paeonia chemistry
Phytotherapy
Plaque, Atherosclerotic blood
Plaque, Atherosclerotic drug therapy
RNA, Messenger genetics
RNA, Messenger metabolism
Tumor Necrosis Factor-alpha blood
Aorta pathology
Apolipoproteins E deficiency
Drugs, Chinese Herbal therapeutic use
Plant Roots chemistry
Plaque, Atherosclerotic enzymology
Plaque, Atherosclerotic pathology
Receptors, IgG metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1993-0402
- Volume :
- 17
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Chinese journal of integrative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 21611899
- Full Text :
- https://doi.org/10.1007/s11655-011-0726-y