Regulation of the Na+/K+ ATPase by Klotho.

Authors :: Sopjani M
Alesutan I
Dërmaku-Sopjani M
Gu S
Zelenak C
Munoz C
Velic A
Föller M
Rosenblatt KP
Kuro-o M
Lang F
Source :: FEBS letters [FEBS Lett] 2011 Jun 23; Vol. 585 (12), pp. 1759-64. Date of Electronic Publication: 2011 May 19.
Publication Year :: 2011
Abstract: Klotho-hypomorphic (Klotho(hm)) mice suffer from renal salt wasting and hypovolemia despite hyperaldosteronism. The present study explored the effect of Klotho on renal Na(+)/K(+) ATPase activity. According to immunohistochemistry and confocal microscopy Na(+)/K(+) ATPase protein abundance in isolated collecting ducts was lower in Klotho(hm) mice than in their wild type littermates (Klotho(+/+)). Analysis with dual electrode voltage clamp recording showed that expression of Klotho in Xenopus oocytes increased the Na(+)/K(+) ATPase pump current. Treatment of Xenopus oocytes with Klotho protein similarly increased the pump current. In conclusion, Klotho increases the membrane abundance and activity of the Na(+)/K(+) ATPase. Decreased Na(+)/K(+) ATPase activity could thus contribute to the volume-depletion of klotho(hm) mice.<br /> (Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)