Back to Search
Start Over
Production and characterization of interspecific somatic hybrids between Brassica oleracea var. botrytis and B. nigra and their progenies for the selection of advanced pre-breeding materials.
- Source :
-
Plant cell reports [Plant Cell Rep] 2011 Oct; Vol. 30 (10), pp. 1811-21. Date of Electronic Publication: 2011 May 21. - Publication Year :
- 2011
-
Abstract
- Somatic hybridization is a potential method for gene transfer from wild relatives to cultivated crops that can overcome sexual incompatibilities of two distantly related species. In this study, interspecific asymmetric somatic hybrids of Brassica oleracea var. botrytis (cauliflower) and Brassica nigra (black mustard) were obtained by protoplast fusion and their backcrossed (BC(3)) and selfed (S(3)) offspring were analyzed. Cytological analysis showed that the B. nigra chromosomes were successively eliminated in the backcrosses with cauliflower. The fertility of the hybrid progenies was quite different due to the asynchronous and abnormal chromosome behavior of pollen mother cells (PMC) during meiosis. Analysis of sequence-related amplified polymorphism (SRAP) showed that all of these hybrids mainly had the DNA banding pattern from the two parents with some alterations. Genetically, the selfed generations were closer to B. nigra, while the backcrossed generations were closer to the cauliflower parent. Analysis of cleaved amplified polymorphic sequences (CAPS) and restriction fragment length polymorphisms (RFLP) showed that all somatic hybrids in this study contained chloroplast (cp) DNA of the donor parent black mustard, while mitochondrial (mt) DNA showed evidence of recombination and variations in the regions analyzed. Furthermore, three BC(3) plants (originated from somatic hybrids 3, 4, 10) with 2-8 B. nigra-derived chromosomes shown by genomic in situ hybridization (GISH) displayed a more cauliflower-like morphology and high resistance to black-rot. These plants were obtained as bridge materials for further analysis and breeding.
- Subjects :
- Brassica physiology
Chromosomes, Plant
DNA, Chloroplast genetics
DNA, Mitochondrial genetics
DNA, Plant genetics
Disease Resistance
Meiosis
Mustard Plant physiology
Pollen cytology
Pollen physiology
Polymorphism, Restriction Fragment Length
Protoplasts cytology
Sequence Analysis, DNA
Xanthomonas campestris pathogenicity
Brassica genetics
Breeding methods
Hybridization, Genetic
Mustard Plant genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-203X
- Volume :
- 30
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 21603996
- Full Text :
- https://doi.org/10.1007/s00299-011-1088-9