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S-adenosyl-L-methionine activates actinorhodin biosynthesis by increasing autophosphorylation of the Ser/Thr protein kinase AfsK in Streptomyces coelicolor A3(2).
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2011; Vol. 75 (5), pp. 910-3. Date of Electronic Publication: 2011 May 20. - Publication Year :
- 2011
-
Abstract
- S-Adenosyl-L-methionine (SAM) is one of the major methyl donors in all living organisms. The exogenous treatment with SAM leads to increased actinorhodin production in Streptomyces coelicolor A3(2). In this study, mutants from different stages of the AfsK-AfsR signal transduction cascade were used to test the possible target of SAM. SAM had no significant effect on actinorhodin production in afsK, afsR, afsS, or actII-open reading frame 4 (ORF4) mutant. This confirms that afsK plays a critical role in delivering the signal generated by exogenous SAM. The afsK-pHJL-KN mutant did not respond to SAM, suggesting the involvement of the C-terminal of AfsK in binding with SAM. SAM increased the in vitro autophosphorylation of kinase AfsK in a dose-dependent manner, and also abolished the effect of decreased actinorhodin production by a Ser/Thr kinase inhibitor, K252a. In sum, our results suggest that SAM activates actinorhodin biosynthesis in S. coelicolor M130 by increasing the phosphorylation of protein kinase AfsK.
- Subjects :
- Anthraquinones metabolism
Bacterial Proteins genetics
Bacterial Proteins metabolism
Carbazoles antagonists & inhibitors
Carbazoles pharmacology
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
Indole Alkaloids antagonists & inhibitors
Indole Alkaloids pharmacology
Mutation
Phosphorylation drug effects
Protein Kinase Inhibitors antagonists & inhibitors
Protein Kinase Inhibitors pharmacology
Protein Serine-Threonine Kinases antagonists & inhibitors
Protein Serine-Threonine Kinases genetics
Signal Transduction drug effects
Streptomyces coelicolor cytology
Streptomyces coelicolor enzymology
Streptomyces coelicolor genetics
Transcription Factors genetics
Transcription Factors metabolism
Protein Serine-Threonine Kinases metabolism
S-Adenosylmethionine pharmacology
Streptomyces coelicolor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 75
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 21597198
- Full Text :
- https://doi.org/10.1271/bbb.100873