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C reactive protein and alpha1-antitrypsin: relationship between levels and gene variants.
- Source :
-
Translational research : the journal of laboratory and clinical medicine [Transl Res] 2011 Jun; Vol. 157 (6), pp. 332-8. Date of Electronic Publication: 2011 Jan 27. - Publication Year :
- 2011
-
Abstract
- The first step in laboratory diagnosis of alpha1-antitrypsin deficiency (AATD) is the determination of alpha1-antitrypsin (AAT) serum levels; these levels in turn are influenced by the inflammatory status. C reactive protein (CRP) has been proposed as a marker of systemic inflammation. Single nucleotide polymorphisms (SNPs) in the CRP gene have been associated with differences in baseline CRP levels. The purpose of this study was to investigate the relationship between CRP and AAT in the AATD diagnostic setting and to verify whether variations in the CRP gene could influence CRP. We determined AAT and CRP levels in 362 consecutive dried blood spot (DBS) samples submitted for AATD diagnosis and genotyped 3 CRP gene SNPs (rs1205, rs3093077, and rs3091244) associated with variations in serum CRP concentrations. To this aim, we developed a method to measure CRP in a DBS with a good correlation with CRP measurement in serum (r2=0.9927). We showed then that systemic inflammatory status parallels increased levels of AAT (80% of subjects with intermediate AATD and a CRP>0.8 mg/dL had an AAT level above the cut-off of 113 mg/dL) and that this increase might mask the presence of AATD variants. No association was detected between CRP levels and the 3 CRP gene polymorphisms. Simultaneous determination of CRP and AAT is useful in the correct diagnosis of heterozygotes carrying intermediate AATD genotypes; their genetic influence on the CRP level is negligible.<br /> (Copyright © 2011 Mosby, Inc. All rights reserved.)
- Subjects :
- Biomarkers blood
Blood Chemical Analysis methods
Genotype
Heterozygote
Humans
Inflammation Mediators blood
Polymorphism, Single Nucleotide
Pulmonary Disease, Chronic Obstructive blood
Pulmonary Disease, Chronic Obstructive genetics
Reference Values
Translational Research, Biomedical
alpha 1-Antitrypsin Deficiency blood
alpha 1-Antitrypsin Deficiency diagnosis
alpha 1-Antitrypsin Deficiency genetics
C-Reactive Protein genetics
C-Reactive Protein metabolism
Genetic Variation
alpha 1-Antitrypsin blood
alpha 1-Antitrypsin genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1810
- Volume :
- 157
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Translational research : the journal of laboratory and clinical medicine
- Publication Type :
- Academic Journal
- Accession number :
- 21575917
- Full Text :
- https://doi.org/10.1016/j.trsl.2010.12.014