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Commensal Bacteroides species induce colitis in host-genotype-specific fashion in a mouse model of inflammatory bowel disease.
- Source :
-
Cell host & microbe [Cell Host Microbe] 2011 May 19; Vol. 9 (5), pp. 390-403. - Publication Year :
- 2011
-
Abstract
- The intestinal microbiota is important for induction of inflammatory bowel disease (IBD). IBD is associated with complex shifts in microbiota composition, but it is unclear whether specific bacterial subsets induce IBD and, if so, whether their proportions in the microbiota are altered during disease. Here, we fulfilled Koch's postulates in host-genotype-specific fashion using a mouse model of IBD with human-relevant disease-susceptibility mutations. From screening experiments we isolated common commensal Bacteroides species, introduced them into antibiotic-pretreated mice, and quantitatively reisolated them in culture. The bacteria colonized IBD-susceptible and -nonsusceptible mice equivalently, but induced disease exclusively in susceptible animals. Conversely, commensal Enterobacteriaceae were >100-fold enriched during spontaneous disease, but an Enterobacteriaceae isolate failed to induce disease in antibiotic-pretreated mice despite robust colonization. We thus demonstrate that IBD-associated microbiota alterations do not necessarily reflect underlying disease etiology. These findings establish important experimental criteria and a conceptual framework for understanding microbial contributions to IBD.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Colitis microbiology
DNA, Bacterial chemistry
DNA, Bacterial genetics
Disease Models, Animal
Gastrointestinal Tract microbiology
Gastrointestinal Tract pathology
Genotype
Histocytochemistry
Inflammatory Bowel Diseases microbiology
Mice
Molecular Sequence Data
Sequence Analysis, DNA
Bacteroides immunology
Bacteroides pathogenicity
Colitis genetics
Colitis pathology
Inflammatory Bowel Diseases genetics
Inflammatory Bowel Diseases pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1934-6069
- Volume :
- 9
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Cell host & microbe
- Publication Type :
- Academic Journal
- Accession number :
- 21575910
- Full Text :
- https://doi.org/10.1016/j.chom.2011.04.009