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The balance of pro-inflammatory and trophic factors in multiple sclerosis patients: effects of acute relapse and immunomodulatory treatment.
- Source :
-
Multiple sclerosis (Houndmills, Basingstoke, England) [Mult Scler] 2011 Jul; Vol. 17 (7), pp. 851-66. Date of Electronic Publication: 2011 May 11. - Publication Year :
- 2011
-
Abstract
- Background: In multiple sclerosis inflammation is primarily injurious to the central nervous system, but its therapeutic suppression might inhibit repair-promoting factors.<br />Objectives: We aimed at better describing the complexity of biological effects during an acute relapse and analysed the effects of intervention with high-dose i.v. glucocorticoids and immunomodulatory treatment with interferon-beta (IFNβ).<br />Methods: We studied the intracellular expression levels of the pro-inflammatory mediators tumour necrosis factor alpha (TNFα) and inducible nitric oxide synthase (iNOS) together with the neurotrophins ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) in freshly isolated peripheral blood mononuclear cells of multiple sclerosis patients during an acute relapse, after intervention with i.v. methylprednisolone and at baseline, using a highly quantitative flow-cytometric approach.<br />Results: We demonstrated the expression of CNTF in human leucocytes. We showed that CNTF levels differed in acutely relapsing multiple sclerosis patients compared with controls and increased after corticosteroid treatment. CNTF can counteract the toxicity of TNFα towards oligodendrocytes and we found TNFα increased during acute relapses. Following corticosteroids, neither TNFα nor iNOS expression was reduced. Levels of BDNF were not affected by glucocorticoids, but increased during IFNβ therapy. However, IFNβ also increased the expression of iNOS and major histocompatibility complex class I (MHC-I), underlining its immunomodulatory potential.<br />Conclusions: Multiple sclerosis patients might benefit from reparative, and not solely from anti-inflammatory, effects of glucocorticoids. Interactive effects of glucocorticoid- and IFNβ-treatment need to be considered to improve neuroprotection and remyelination resulting from immunomodulatory treatment.
- Subjects :
- Adult
Brain-Derived Neurotrophic Factor blood
Case-Control Studies
Cell Separation methods
Cells, Cultured
Ciliary Neurotrophic Factor blood
Drug Therapy, Combination
Female
Flow Cytometry
Humans
Interferon beta-1a
Interferon beta-1b
Leukocytes, Mononuclear immunology
Leukocytes, Mononuclear metabolism
Male
Middle Aged
Multiple Sclerosis, Chronic Progressive blood
Multiple Sclerosis, Chronic Progressive diagnosis
Multiple Sclerosis, Chronic Progressive immunology
Multiple Sclerosis, Relapsing-Remitting blood
Multiple Sclerosis, Relapsing-Remitting diagnosis
Multiple Sclerosis, Relapsing-Remitting immunology
Nitric Oxide Synthase Type II blood
Treatment Outcome
Tumor Necrosis Factor-alpha blood
Young Adult
Glucocorticoids administration & dosage
Immunologic Factors therapeutic use
Inflammation Mediators blood
Interferon-beta therapeutic use
Leukocytes, Mononuclear drug effects
Methylprednisolone administration & dosage
Multiple Sclerosis, Chronic Progressive drug therapy
Multiple Sclerosis, Relapsing-Remitting drug therapy
Nerve Growth Factors blood
Subjects
Details
- Language :
- English
- ISSN :
- 1477-0970
- Volume :
- 17
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Multiple sclerosis (Houndmills, Basingstoke, England)
- Publication Type :
- Academic Journal
- Accession number :
- 21561957
- Full Text :
- https://doi.org/10.1177/1352458511399797