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High-throughput total cupric ion reducing antioxidant capacity of biological samples determined using flow injection analysis and microplate-based methods.
- Source :
-
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2011; Vol. 27 (5), pp. 483. - Publication Year :
- 2011
-
Abstract
- High-throughput cupric ion reducing antioxidant capacity (CUPRAC) methods were developed for assessment of total antioxidant capacity (TAC) in urine and serum, based on reduction of Cu(II)-neocuproine complex to highly colored Cu(I)-neocuproine complex, measured spectrophotometrically at 450 nm. The reaction time was significantly reduced from 30 to 4 min by application of a calibration compound (uric acid) with kinetic behavior similar to that shown by urine samples. The method was implemented in a microformat (96 well plates) and also in an automatic fashion (flow injection analysis, FIA). A determination throughput value of 288 h(-1) (microplate method) or of 15 h(-1) (automatic FIA) was attained. Application of both methods to human serum (SRM 909b, level I) and urines (n = 9) provided TAC values in agreement with those of the end-point batch method.
Details
- Language :
- English
- ISSN :
- 1348-2246
- Volume :
- 27
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 21558653
- Full Text :
- https://doi.org/10.2116/analsci.27.483