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Phenotypic variation of fluoride responses between inbred strains of mice.
- Source :
-
Cells, tissues, organs [Cells Tissues Organs] 2011; Vol. 194 (2-4), pp. 261-7. Date of Electronic Publication: 2011 May 09. - Publication Year :
- 2011
-
Abstract
- Excessive systemic exposure to fluoride (F) can lead to disturbances in bone homeostasis and dental enamel development. We have previously shown strain-specific responses to F in the development of dental fluorosis (DF) and in bone formation/mineralization. The current study was undertaken to further investigate F responsive variations in bone metabolism and to determine possible relationships with DF susceptibility. Seven-week-old male mice from FVB/NJ, C57BL/6J, C3H/HeJ, A/J, 129S1/SvImJ, AKR/J, DBA/2J, and BALB/cByJ inbred strains were exposed to NaF (0 or 50 ppm as F(-)) in drinking water for 60 days. Sera were collected for F, Ca, Mg, PO(4), iPTH, sRANKL, and ALP levels. Bone marrow cells were subjected to ex vivo cell culture for osteoclast potential and CFU colony assays (CFU-fibroblast, CFU-osteoblast, CFU-erythrocyte/granulocyte/macrophage/megakaryocyte, CFU-granulocyte/macrophage, CFU-macrophage, and CFU-granulocyte). Femurs and vertebrae were subjected to micro-CT analyses, biomechanical testing, and F, Mg, and Ca content assays. DF was evaluated using quantitative fluorescence and clinical criteria. Strain-specific responses to F were observed for DF, serum studies, ex vivo cell culture studies, and bone quality. Among the strains, there were no patterns or significant correlations between DF severity and the actions of F on bone homeostasis (serum studies, ex vivo assays, or bone quality parameters). The genetic background continues to play a role in the actions of F on tooth enamel development and bone homeostasis. F exposure led to variable phenotypic responses between strains involving dental enamel development and bone metabolism.<br /> (Copyright © 2011 S. Karger AG, Basel.)
- Subjects :
- Animals
Biomechanical Phenomena
Bone Marrow Cells metabolism
Bone and Bones diagnostic imaging
Bone and Bones physiopathology
Cells, Cultured
Enzyme-Linked Immunosorbent Assay
Fluorescence
Fluorosis, Dental blood
Fluorosis, Dental diagnostic imaging
Fluorosis, Dental pathology
Fluorosis, Dental physiopathology
Male
Mice
Mice, Inbred Strains
Phenotype
Spine diagnostic imaging
Spine drug effects
Spine pathology
Spine physiopathology
X-Ray Microtomography
Bone and Bones drug effects
Bone and Bones pathology
Fluorides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-6421
- Volume :
- 194
- Issue :
- 2-4
- Database :
- MEDLINE
- Journal :
- Cells, tissues, organs
- Publication Type :
- Academic Journal
- Accession number :
- 21555858
- Full Text :
- https://doi.org/10.1159/000324224