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Proteomic approach for the detection of chicken mechanically recovered meat.
- Source :
-
Meat science [Meat Sci] 2011 Oct; Vol. 89 (2), pp. 233-7. Date of Electronic Publication: 2011 Apr 15. - Publication Year :
- 2011
-
Abstract
- Mechanically recovered meat is cheaper than raw meat and thus has been incorporated into many meat-derived products. EU regulations exclude mechanically recovered meat from the definition of meat; as a consequence analytical procedures are needed to differentiate it from hand-deboned meat. The present pilot study has utilized a proteomic approach to find potential markers for the detection of chicken mechanically recovered meat. Intact proteins were extracted from raw meat and then analyzed with OFF-GEL electrophoresis followed by SDS-PAGE and identification of potential markers by nano-LC-MS/MS. It was shown that it is possible to extract, separate and identify key proteins from processed meat material. Potential chicken mechanically recovered meat markers--hemoglobin subunits and those similar to myosin-binding protein C were also identified.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Biomarkers analysis
Biomarkers chemistry
Carrier Proteins isolation & purification
Chickens
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Food Analysis methods
Hemoglobin Subunits isolation & purification
Mass Spectrometry
Pilot Projects
Food Handling methods
Meat analysis
Proteomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4138
- Volume :
- 89
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Meat science
- Publication Type :
- Academic Journal
- Accession number :
- 21555191
- Full Text :
- https://doi.org/10.1016/j.meatsci.2011.04.004