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Reduced dosage of the modifiers of epigenetic reprogramming Dnmt1, Dnmt3L, SmcHD1 and Foxo3a has no detectable effect on mouse telomere length in vivo.
- Source :
-
Chromosoma [Chromosoma] 2011 Aug; Vol. 120 (4), pp. 377-85. Date of Electronic Publication: 2011 May 07. - Publication Year :
- 2011
-
Abstract
- Studies carried out in cultured cells have implicated modifiers of epigenetic reprogramming in the regulation of telomere length, reporting elongation in cells that were null for DNA methyltransferase DNA methyltransferase 1 (Dnmt1), both de novo DNA methyltransferases, Dnmt3a and Dnmt3b or various histone methyltransferases. To investigate this further, we assayed telomere length in whole embryos or adult tissue from mice carrying mutations in four different modifiers of epigenetic reprogramming: Dnmt1, DNA methyltransferase 3-like, structural maintenance of chromosomes hinge domain containing 1, and forkhead box O3a. Terminal restriction fragment analysis was used to compare telomere length in homozygous mutants, heterozygous mutants and wild-type littermates. Contrary to expectation, we did not detect overall lengthening in the mutants, raising questions about the role of epigenetic processes in telomere length in vivo.
- Subjects :
- Animals
Chromosomal Proteins, Non-Histone deficiency
DNA (Cytosine-5-)-Methyltransferase 1
DNA (Cytosine-5-)-Methyltransferases deficiency
DNA Methylation
Electrophoresis, Agar Gel
Embryo, Mammalian
Female
Forkhead Box Protein O3
Forkhead Transcription Factors deficiency
Gene Dosage
Histone Methyltransferases
Histone-Lysine N-Methyltransferase genetics
Histone-Lysine N-Methyltransferase metabolism
Mice
Mice, Inbred C57BL
Mice, Transgenic
Mutation
Pregnancy
Restriction Mapping
Telomere genetics
Chromosomal Proteins, Non-Histone genetics
DNA (Cytosine-5-)-Methyltransferases genetics
Epigenomics
Forkhead Transcription Factors genetics
Telomere chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0886
- Volume :
- 120
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Chromosoma
- Publication Type :
- Academic Journal
- Accession number :
- 21553025
- Full Text :
- https://doi.org/10.1007/s00412-011-0318-9