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Biomarker discovery and proteomic evaluation of cadmium toxicity on a collembolan species, Paronychiurus kimi (Lee).
- Source :
-
Proteomics [Proteomics] 2011 Jun; Vol. 11 (11), pp. 2294-307. Date of Electronic Publication: 2011 May 05. - Publication Year :
- 2011
-
Abstract
- The goal of this study was to identify promising new biomarkers of cadmium by identifying differentially expressed proteins in Paronychiurus kimi after exposure to cadmium. Through proteomic analysis of P. kimi using 1-D PAGE and nano-LC-MS/MS, 36 downregulated proteins and 40 upregulated proteins were found. Some of the downregulated and upregulated proteins were verified by LC-MS/MS analysis after 2-D PAGE. Downregulated proteins in response to cadmium exposure were involved in glycolysis and energy metabolism, chaperones, transcription, reproduction, and neuron growth. In contrast, proteins involved in glycolysis and energy production, neurogenesis, defense systems response to bacteria, and protein biosynthesis were upregulated in cadmium-treated collembolans. Cubulin may be a potential biomarker for the detection of cadmium in P. kimi since this biomarker was able to low levels (3.5 mg/kg) of cadmium. The 14-3-3 ζ was also found to be a potential biomarker for the detection of medium levels (14 mg/kg) of cadmium. Collembolans may be an alternative tool to humans because many collembolans proteins show a high homology to human proteins.<br /> (Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Analysis of Variance
Animals
Arthropods physiology
Biomarkers analysis
Biomarkers chemistry
Chromatography, Liquid
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Insect Proteins analysis
Insect Proteins classification
Insect Proteins metabolism
Proteome chemistry
Receptors, Cell Surface
Tandem Mass Spectrometry
Arthropods drug effects
Cadmium toxicity
Insect Proteins chemistry
Proteome drug effects
Proteomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1615-9861
- Volume :
- 11
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 21548089
- Full Text :
- https://doi.org/10.1002/pmic.200900690