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Acoustically mounted microcrystals yield high-resolution X-ray structures.
- Source :
-
Biochemistry [Biochemistry] 2011 May 31; Vol. 50 (21), pp. 4399-401. Date of Electronic Publication: 2011 May 09. - Publication Year :
- 2011
-
Abstract
- We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 วบ resolution crystal structures.
- Subjects :
- Acoustics
Crystallography, X-Ray methods
Subjects
Details
- Language :
- English
- ISSN :
- 1520-4995
- Volume :
- 50
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 21542590
- Full Text :
- https://doi.org/10.1021/bi200549x