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Impact of maturational status on the ability of osteoblasts to enhance the hematopoietic function of stem and progenitor cells.
- Source :
-
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research [J Bone Miner Res] 2011 May; Vol. 26 (5), pp. 1111-21. - Publication Year :
- 2011
-
Abstract
- Osteoblasts (OBs) exert a prominent regulatory effect on hematopoietic stem cells (HSCs). We evaluated the difference in hematopoietic expansion and function in response to co-culture with OBs at various stages of development. Murine calvarial OBs were seeded directly (fresh) or cultured for 1, 2, or 3 weeks prior to seeding with 1000 Lin-Sca1 + cKit+ (LSK) cells for 1 week. Significant increases in the following hematopoietic parameters were detected when comparing co-cultures of fresh OBs to co-cultures containing OBs cultured for 1, 2, or 3 weeks: total hematopoietic cell number (up to a 3.4-fold increase), total colony forming unit (CFU) number in LSK progeny (up to an 18.1-fold increase), and percentage of Lin-Sca1+ cells (up to a 31.8-fold increase). Importantly, these studies were corroborated by in vivo reconstitution studies in which LSK cells maintained in fresh OB co-cultures supported a significantly higher level of chimerism than cells maintained in co-cultures containing 3-week OBs. Characterization of OBs cultured for 1, 2, or 3 weeks with real-time PCR and functional mineralization assays showed that OB maturation increased with culture duration but was not affected by the presence of LSK cells in culture. Linear regression analyses of multiple parameters measured in these studies show that fresh, most likely more immature OBs better promote hematopoietic expansion and function than cultured, presumably more mature OBs and suggest that the hematopoiesis-enhancing activity is mediated by cells present in fresh OB cultures de novo.<br /> (Copyright © 2011 American Society for Bone and Mineral Research.)
- Subjects :
- Animals
Apoptosis
Calcium metabolism
Cell Count
Cell Cycle
Cell Lineage
Cell Proliferation
Cell Separation
Cells, Cultured
Coculture Techniques
Colony-Forming Units Assay
Gene Expression Regulation
Mice
Mice, Inbred C57BL
Phenotype
RNA, Messenger genetics
RNA, Messenger metabolism
Cell Differentiation
Hematopoiesis physiology
Hematopoietic Stem Cells cytology
Hematopoietic Stem Cells metabolism
Osteoblasts cytology
Osteoblasts metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1523-4681
- Volume :
- 26
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
- Publication Type :
- Academic Journal
- Accession number :
- 21542011
- Full Text :
- https://doi.org/10.1002/jbmr.302