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Genetic diversity of Lactobacillus paracasei isolated from in situ human oral biofilms.

Authors :
Parolo CC
Do T
Henssge U
Alves LS
de Santana Giongo FC
Corção G
Maltz M
Beighton D
Source :
Journal of applied microbiology [J Appl Microbiol] 2011 Jul; Vol. 111 (1), pp. 105-13. Date of Electronic Publication: 2011 May 20.
Publication Year :
2011

Abstract

Aim: To determine the genetic diversity and possible origin of Lactobacillus paracasei found in the oral biofilm.<br />Methods and Results: Lactobacilli were isolated from a biofilm model, formed in situ prior to and during a period of exposure to 20% sucrose solution (28 days), using Rogosa Agar. The lactobacillus colonies were randomly selected (n = 222) and subcultured. The isolates were identified using pheS or rpoA gene sequence analysis. Lactobacilli identified as Lact. paracasei (n = 75) were subjected to multilocus sequencing typing (MLST) analysis by determining partial sequences of seven housekeeping genes fusA, ileS, lepA, leuS, pyrG, recA and recG. An increase recovery of lactobacilli after sucrose phase compared with nonsucrose period was observed (31 prior to and 191 following a sucrose exposure period). Seven subjects harboured Lact. paracasei and these represented 14 sequence types (ST). Comparison of the STs showed that unrelated subjects may harbour the same ST and that individuals harbour multiple STs. Three subjects harboured STs previously isolated from dairy products.<br />Conclusion: The present data supports the hypothesis that oral lactobacilli may be of exogenous origin.<br />Significance and Impact of the Study: The study allow us to gain insight into the genetic diversity of Lact. paracasei in oral biofilm.<br /> (Journal of Applied Microbiology © 2011 The Society for Applied Microbiology. No claim to Brazilian Government works.)

Details

Language :
English
ISSN :
1365-2672
Volume :
111
Issue :
1
Database :
MEDLINE
Journal :
Journal of applied microbiology
Publication Type :
Academic Journal
Accession number :
21535332
Full Text :
https://doi.org/10.1111/j.1365-2672.2011.05041.x