An ultrasensitive post chemiluminescence reaction of ammonium in NBS-dichlorofluorescein system and its application.

A strong post chemiluminescence (PCL) phenomenon was observed when ammonium was injected into the reaction mixture after the finish of CL reaction of N-bromosuccinimide (NBS) and dichlorofluorescein. Based on this, a sensitive flow injection PCL method was established for the determination of ammonium. The possible CL mechanism of the reaction was proposed based on a series of experiments. The PCL intensity responded linearly to the concentration of ammonium in the range 3.0 × 10(-11) -1.0 × 10(-7) g mL(-1) with a detection limit of 1 × 10(-11) g mL(-1). The relative standard deviation (R.S.D.) was 1.4% for 1.0 × 10(-9) g mL(-1) ammonium (n=11). This method had been applied to the determination of ammonium in samples of mineral water, tap water and river water.
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