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Krüppel-like factor 2 regulated gene expression in mouse embryonic yolk sac erythroid cells.
- Source :
-
Blood cells, molecules & diseases [Blood Cells Mol Dis] 2011 Jun 15; Vol. 47 (1), pp. 1-11. Date of Electronic Publication: 2011 Apr 29. - Publication Year :
- 2011
-
Abstract
- KLF2 is a Krüppel-like zinc-finger transcription factor required for blood vessel, lung, T-cell and erythroid development. KLF2-/- mice die by embryonic day 14.5 (E14.5), due to hemorrhaging and heart failure. In KLF2-/- embryos, β-like globin gene expression is reduced, and E10.5 erythroid cells exhibit abnormal morphology. In this study, other genes regulated by KLF2 were identified by comparing E9.5 KLF2-/- and wild-type (WT) yolk sac erythroid precursor cells, using laser capture microdissection and microarray assays. One hundred and ninety-six genes exhibited significant differences in expression between KLF2-/- and WT; eighty-nine of these are downregulated in KLF2-/-. Genes involved in cell migration, differentiation and development are over-represented in the KLF2-regulated gene list. The SOX2 gene, encoding a pluripotency factor, is regulated by KLF2 in both ES and embryonic erythroid cells. Previous work had identified genes with erythroid-enriched expression in the yolk sac. The erythroid-enriched genes reelin, adenylate cyclase 7, cytotoxic T lymphocyte-associated protein 2 alpha, and CD24a antigen are downregulated in KLF2-/- compared to WT and are therefore candidates for controlling primitive erythropoiesis. Each of these genes contains a putative KLF2 binding site(s) in its promoter and/or an intron. Reelin has an established role in neuronal development. Luciferase reporter assays demonstrated that KLF2 directly transactivates the reelin promoter in erythroid cells, validating this approach to identify KLF2 target genes.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Adhesion Molecules, Neuronal genetics
Cell Line
Extracellular Matrix Proteins genetics
Female
Gene Expression Profiling
Gene Regulatory Networks genetics
Humans
K562 Cells
Male
Mice
Mice, Knockout
Nerve Tissue Proteins genetics
Promoter Regions, Genetic genetics
Reelin Protein
Serine Endopeptidases genetics
Yolk Sac cytology
Erythroid Cells metabolism
Gene Expression Regulation, Developmental
Kruppel-Like Transcription Factors genetics
Kruppel-Like Transcription Factors metabolism
Yolk Sac embryology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0961
- Volume :
- 47
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Blood cells, molecules & diseases
- Publication Type :
- Academic Journal
- Accession number :
- 21530336
- Full Text :
- https://doi.org/10.1016/j.bcmd.2011.03.002