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Unraveling the role of fermentation in the mode of action of acetolactate synthase inhibitors by metabolic profiling.
- Source :
-
Journal of plant physiology [J Plant Physiol] 2011 Sep 01; Vol. 168 (13), pp. 1568-75. Date of Electronic Publication: 2011 Apr 29. - Publication Year :
- 2011
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Abstract
- Herbicides that inhibit branched chain amino acid biosynthesis induce aerobic fermentation. The role of fermentation in the mode of action of these herbicides is not known, nor is the importance of this physiological response in the growth inhibition and the lethality caused by them. Metabolic profiling was used to compare the effects of the herbicide imazethapyr (IM) on pea plants with two other treatments that also induce fermentation: hypoxia and the exogenous supply pyruvate for seven days. While hypoxic roots did not show internal anoxia, feeding pyruvate or applying IM to the roots led to internal anoxia, probably related to the respiratory burst detected. The three treatments induced ethanol fermentation, but fermentation induced following herbicide treatment was earlier than that following pyruvate supply and was not associated with a decrease in the energy status. No striking changes were detected in the metabolic profiling of hypoxic roots, indicating that metabolism was only slightly impaired. Feeding pyruvate resulted in marked succinate accumulation and a general amino acid accumulation. IM-treated roots showed a general accumulation of glycolytic metabolites upstream of pyruvate, a decrease in some TCA intermediates and an increase in the free amino acid pool sizes. All treatments caused GABA and putrescine accumulation. Our results indicate that IM supply impairs carbon/nitrogen metabolism and this impaired metabolism is likely to be related to the growth arrest detected. As growth is arrested, carbohydrates and glycolytic intermediates accumulate and energy becomes more available.<br /> (Copyright © 2011 Elsevier GmbH. All rights reserved.)
- Subjects :
- Acetolactate Synthase antagonists & inhibitors
Amino Acids metabolism
Amino Acids, Branched-Chain antagonists & inhibitors
Amino Acids, Branched-Chain biosynthesis
Cell Hypoxia physiology
Fermentation physiology
Gas Chromatography-Mass Spectrometry
Oxidation-Reduction
Oxygen analysis
Pisum sativum enzymology
Pisum sativum growth & development
Plant Roots growth & development
Plant Roots metabolism
Plant Shoots growth & development
Plant Shoots metabolism
Putrescine metabolism
Pyruvic Acid pharmacology
Time Factors
gamma-Aminobutyric Acid metabolism
Acetolactate Synthase metabolism
Fermentation drug effects
Herbicides pharmacology
Nicotinic Acids pharmacology
Oxygen metabolism
Pisum sativum metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1618-1328
- Volume :
- 168
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Journal of plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 21529995
- Full Text :
- https://doi.org/10.1016/j.jplph.2011.02.015