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Detection of norovirus, sapovirus, and human astrovirus in fecal specimens using a multiplex reverse transcription-PCR with fluorescent dye-labeled primers.
- Source :
-
Microbiology and immunology [Microbiol Immunol] 2011 May; Vol. 55 (5), pp. 369-72. - Publication Year :
- 2011
-
Abstract
- We applied a multiplex reverse transcription-PCR with fluorescent dye-labeled primers (fluorescent multiplex RT-PCR) for noroviruses (NoV), sapovirus (SaV), and human astrovirus (HAstV) to diagnose 71 outbreaks of acute gastroenteritis during July 2007 and May 2010 in Hiroshima prefecture. In this assay, the green, red, yellow, and blue fluorescence for NoV genogroup I, NoV genogroup II, SaV, and HAstV, respectively, were indicated on an agarose gel under ultraviolet light. In 61 virus-positive outbreaks confirmed by fluorescent multiplex RT-PCR, detection rates of outbreaks for NoVs, SaV, and HAstV were 96.7%, 3.3%, and 0%, respectively.<br /> (© 2011 The Societies and Blackwell Publishing Asia Pty Ltd.)
- Subjects :
- DNA Primers
Disease Outbreaks
Fluorescent Dyes
Gastroenteritis epidemiology
Gastroenteritis virology
Humans
Japan
Mamastrovirus genetics
Norovirus genetics
RNA, Viral analysis
RNA, Viral isolation & purification
Sapovirus genetics
Feces virology
Gastroenteritis diagnosis
Mamastrovirus isolation & purification
Norovirus isolation & purification
Reverse Transcriptase Polymerase Chain Reaction methods
Sapovirus isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1348-0421
- Volume :
- 55
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Microbiology and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 21517947
- Full Text :
- https://doi.org/10.1111/j.1348-0421.2011.00325.x